surveillance of airborne adenovirus and mycoplasma pneumoniae in a hospital pediatric department监视空中腺病毒肺炎支原体在医院儿科部门.pdfVIP

Surveillance of Airborne Adenovirus and Mycoplasma pneumoniae in a Hospital Pediatric Department 1 . 2,3. 4,5 1 2 Gwo-Hwa Wan * , Chung-Guei Huang , Yhu-Chering Huang , Ju-Ping Huang , Su-Li Yang , Tzou- Yien Lin4,5, Kuo-Chien Tsao2,3 1 Department of Respiratory Therapy, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan, 2 Department of Laboratory Medicine, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan, 3 Department of Biotechnology and Laboratory Science, Research Center for Emerging Viral Infections, Chang Gung University, Tao-Yuan, Taiwan, 4 Division of Pediatric Infectious Diseases, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan, 5 College of Medicine, Chang Gung University, Tao-Yuan, Taiwan Abstract This investigation evaluated the distributions of airborne adenovirus and Mycoplasma pneumoniae in public areas in the pediatric department of Children’s Hospital in northern Taiwan. The airborne viral and bacterial concentrations were evaluated twice a week for a year using filter sampling with an airflow rate of 12 liters per minute for eight hours in the pediatric outpatient department and 24 hours in the pediatric emergency room. Real-time polymerase chain reaction assays were conducted for analysis. Approximately 18% of the air samples from the pediatric emergency room were found to contain adenovirus. Approximately forty-six percent of the air samples from the pediatric outpatient department contained Mycoplasma pneumoniae DNA products. High detection rates of airborne adenovirus DNA were obtained in July and August in the pediatric public areas. Airborne Mycoplasma pneumoniae was detected only in July in the pediatric emergency room and the peak