srpx2 is a novel chondroitin sulfate proteoglycan that is overexpressed in gastrointestinal cancersrpx2是一种新型的硫酸软骨素蛋白多糖,在胃肠道癌症.pdfVIP

SRPX2 Is a Novel Chondroitin Sulfate Proteoglycan That Is Overexpressed in Gastrointestinal Cancer 1,2 1 1 1 1 Kaoru Tanaka , Tokuzo Arao , Daisuke Tamura , Keiichi Aomatsu , Kazuyuki Furuta , Kazuko 1 1,2 1 1 1 Matsumoto , Hiroyasu Kaneda , Kanae Kudo , Yoshihiko Fujita , Hideharu Kimura , Kazuyoshi 3 4 2 2 1 Yanagihara , Yasuhide Yamada , Isamu Okamoto , Kazuhiko Nakagawa , Kazuto Nishio * 1 Department of Genome Biology, Kinki University School of Medicine, Osaka-Sayama, Osaka, Japan, 2 Department of Medical Oncology, Kinki University School of Medicine, Osaka-Sayama, Osaka, Japan, 3 Department of Life Science, Faculty of Pharmacy, Yasuda Women’s University, Asaminami-ku, Hiroshima, Japan, 4 Department of Medical Oncology, National Cancer Center Hospital, Chuo-ku, Tokyo, Japan Abstract SRPX2 (Sushi repeat-containing protein, X-linked 2) has recently emerged as a multifunctional protein that is involved in seizure disorders, angiogenesis and cellular adhesion. Here, we analyzed this protein biochemically. SRPX2 protein was secreted with a highly posttranslational modification. Chondroitinase ABC treatment completely decreased the molecular mass of purified SRPX2 protein to its predicted size, whereas heparitinase, keratanase and hyaluroinidase did not. Secreted SRPX2 protein was also detected using an anti-chondroitin sulfate antibody. These results indicate that SRPX2 is a novel chondroitin sulfate proteoglycan (CSPG). Furthermore, a binding assay reveal