养肝利胆颗粒对小鼠四氯化碳肝损伤保护作用.docVIP

养肝利胆颗粒对小鼠四氯化碳肝损伤保护作用 　 作者：尤圣富，郑培永，季光，魏华凤，赵婕，朱培庭 【关键词】 养肝利胆颗粒 ［摘要］ 目的：观察养肝利胆颗粒对小鼠四氯化碳（carbon tetrachloride, CCl4）肝损伤的影响，并探讨其作用机制。方法：采用腹腔注射CCl4制作小鼠慢性肝损伤模型。造模3周后分别予以养肝利胆颗粒小、中、大剂量，胆宁片及联苯双酯治疗4周，检测各组血清丙氨酸氨基转移酶（alanine aminotransferase, ALT），天门冬氨酸氨基转移酶（aspartate aminotransferase, AST），肝组织超氧化物歧化酶（superoxide dismutase, SOD）活性和丙二醛（malondialdehyde, MDA）含量。结果：养肝利胆颗粒可显著降低小鼠CCl4肝损伤模型血清ALT、AST的水平和肝组织MDA的含量，明显升高肝组织SOD的水平。结论：养肝利胆颗粒对CCl4所致小鼠肝损伤有明显的改善作用，其作用机制可能与减轻肝内脂质过氧化反应有关。 　　［关键词］ 养肝利胆颗粒; 四氯化碳; 超氧化物歧化酶; 丙二醛 　　Protective effects of Yanggan Lidan Granules on carbon tetrachlorideinduced liver damage in mice 　　ABSTRACT Objective: To observe the protective effects of Yanggan Lidan Granules (YGLDG) on carbon tetrachloride (CCl4)induced liver damage in mice and to find out its mechanism. Methods: A model of chronic liver damage was established in mice by intraperitoneal injection of CCl4. After three weeks, those model mice were treated with low, medium, highdose YGLDG, Danning Tablets and bifendate respectively for four weeks. Then the levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), as well as the contents of superoxide dismutase (SOD) and malondialdehyde (MDA) in liver tissues were detected. Results: YGLDG could significantly reduce the levels of serum ALT and AST in model mice, and the content of MDA was obviously decreased while the content of SOD was increased in liver tissue. Conclusion: The therapeutic effect of YGLDG on mice with CCl4induced liver damage is to relieve the seriousness of liver damage, and its mechanism may relate to reducing peroxidation activity in liver tissue. 　　KEY WORDS Yanggan Lidan Granules; carbon tetrachloride; superoxide dismutase; malondialdehyde 　　目前，具有保肝、抗肝损伤作用的中药有效成分或复方，如甘草甜素、联苯双酯、当飞利肝宁和黄芪注射液等虽在临床上被广泛应用，但经国际认可的该类药物均非源自我国［1］。养肝利胆颗粒（Yanggan Lidan Granules, YGLDG）具有养肝柔肝、利胆止痛的功效，临床上治疗慢性胆道感染（慢性胆囊炎、慢性胆管炎）证属肝阴不足者有一定的疗效。我们观察了养肝利胆颗粒对小鼠四氯化碳（carbon tetrachloride, CCl4）慢性肝损伤的保护作用，以期为临床用药提供依据并对其作用机制作进一步的探讨。 　　1 材料与方法 　　1.1 药物与