一品红苞片花色素的分离及初步鉴定.pdfVIP

植物学通报 2006, 23 (4): 356~362 Chinese Bulletin of Botany . . 实验简报 一品红苞片花色素的分离及初步鉴定 1* 2 王长泉 魏小兵 (1 山东理工大学生命科学学院 淄博 255049) (2 山东理工大学分析测试中心 淄博 255049) 摘要 用紫外- 可见光分光光度计、高效液相色谱(HPLC) 和质谱(MS) 技术对一品红(Euphorbia pulcherrima )红色苞片中的花色素提取液进行了初步鉴定。一品红花色素的甲醇溶液分别在270 、340 和 520 nm 处有 3 个吸收峰; 在 440 nm 吸光度与可见光最大吸收波长 520 nm 吸光度的比值为0.29; 花色素 的甲醇溶液中加入AlCl 后发生红移, 再加入HCl后发生蓝移; 色素溶液在紫外光下无荧光; 色素样品经液 3 相色谱分离后在 270 nm检测有 5 个比较明显的吸收峰; 质谱中得到 595 、611、381、571 和 589 等对应 的分子离子峰; 花色素酸解液高效液相色谱图谱和鼠李糖、葡萄糖的出峰时间一致。由这些结果可推断 一品红花色素样品中主要含有 5 种组分: 矢车菊花色素芸香苷、飞燕草花色素芸香苷、飞燕草花色素苯 甲酰基葡糖苷、矢车菊花色素苯甲酰基葡糖苷和一种未知成分。 关键词 一品红, 花色素, 紫外-可见光分光光度计, 液相色谱, 质谱 The Extraction and Primary Identification of Anthocyanidin in Euphorbia pulcherrima 1 2 Changquan Wang , Xiaobing Wei (1 College of Life Science, Shandong University of Technology, Zibo 255049) (2 Test and Analysis Center, Shandong University of Technology, Zibo 255049) Abstract Anthocyanidins in Euphorbia pulcherrima were extracted and identified by use of UV-vis spectrophotometry, high performance liquid chromatography (HPLC) and mass spectrometry (MS). Anthocyanidin extract in methanol showed 3 absorption peaks, at 270, 340 and 520 nm, in the UV-vis spectrum, with bathochromic and hypsochromic shift with methanol+AlCl and methanol+AlCl +HCl, 3 3 respectively; the ratio of the absorbance at 440 nm to that at 520 nm was 0.29. No fluorescence occurred under UV irradiance, and 5 absorption peaks occurred at 270 nm after HPLC. Five molecular ion peaks (m/z) occurred on MS: 595, 381, 611, 587 and 571, respectively, and monosaccharides in the extra