pFastBacHT C昆虫表达载体说明.pdfVIP

pFastBacHT C 编号 载体名称 北京华越洋生物KCVECT137 pFastBacHT C pFastBacHTC 载体基本信息 载体名称: pFastBacHTC, pFastBac HTC, pFastBacHT C, pFastBac HT C 质粒类型: 昆虫细胞表达载体 启动子: Polyhedrin 表达水平: 高 克隆方法: 多克隆位点，限制性内切酶 载体大小: 4858 bp 5 测序引物: pFastbac-F: TATTCCGGATTATTCATACC 3 测序引物: pFastBac-R: ACAAATGTGGTATGGCTGA 载体标签: N-His, N-TEV 载体抗性: Ampicillin 氨苄 筛选标记: Gentamicin 庆大霉素 稳定性: 稳表达 Stable 组成型: 组成型 Constitutive 病毒/非病毒: 杆状病毒 pFastBacHTC 载体质粒图谱和多克隆位点信息 pFastBacHTC 载体简介 The pFastBac HT vector is supplied with the multiple cloning site in three reading frames (A, B, and C) to facilitate cloning your gene of interest in frame with the N-terminal 6xHis tag. • Strong polyhedrin (PH) promoter for high-level protein expression. • N-terminal 6xHis tag for purification of recombinant fusion proteins using metal-chelating resin and a TEV protease cleavage site for removal of the 6xHis tag following protein purification. • Vector supplied in 3 reading frames for simplified cloning. pFastBacHTC 载体序列 ORIGIN 1 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 61 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 121 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 181 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 241 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 301 GGACTCTTGT TCCAAACTGG AACAACAC