bFGF及其Mab对侵袭性垂体腺瘤裸鼠皮下移植瘤作用的研究.docVIP

bFGF及其Mab对侵袭性垂体腺瘤裸鼠皮下移植瘤作用的研究 娄四龙1,戴勤弼1,毛德强1,霍 钢2,廖正步2（400030重庆,重庆市肿瘤研究所神经外科1； 400010重庆,重庆医科大学附属临床学院神经外科2） [摘要] 目的 探讨用bFGF及其不同浓度单克隆抗体（bFGF-Mab）的方法对裸鼠人侵袭性垂体腺瘤移植瘤血管生成和肿瘤生长的影响及可能机制。方法 建立50只人侵袭性垂体腺瘤裸鼠皮下移植瘤模型， 按区组随机排列方法分成bFGF组、30、20、lOμg/ml 3个抗体浓度的bFGF-Mab组及生理盐水对照组，每组10只，分别给予不同处理后8周终止实验，每周测量肿瘤体积,绘制移植瘤体积曲线；对移植瘤组织用免疫组化法检测CD31的表达，以计算肿瘤组织微血管密度（microvessel density，MVD）。结果 与对照组、bFGF组比较，bFGF-Mab组对移植瘤的生长及肿瘤血管生成有明显抑制作用，以30μg/ml组的抑瘤率（55.71%）最高（P0.01）；bFGF-Mab各浓度组肿瘤组织中MVD均明显下降，30μg/ml组（3.36±1.26）下降最明显（P0.01）。结论 bFGF-Mab处理后对裸鼠侵袭性垂体腺瘤皮下移植瘤具有较强的体内抑瘤效应，可能是通过抑制细胞增殖活性及肿瘤血管生成而达到抑制垂体腺瘤的侵袭性生长的。 [关键词] 侵袭性垂体腺瘤；bFGF-Mab；抑瘤效应 Experimental study on inhibitory effect of bFGF-Mab on the invasive pituitary adenoma in nude mice Lou Si-long1 ，DAI Qin-bi1，MAO De-qiang1，HUO Gang2，LIAO Zheng-bu2 （1Department of Neurosurgery, The Cancer Institute of Chongqing, Chongqing 400030； 2 Department of Neurosurgery, The First Affiliated Hospital ,Chongqing Medical University , Chongqing 400016, China） [Abstract] Objective To investigate the inhibitory effect of the monoclonal antibody of basic fibroblast growth factor(bFGF-Mab) on nude micetransplanted human invasive pituitary adenoma and its possible mechanism of action. Methods we established nude micetransplanted human invasive pituitary adenoma .Then mice were randomly into 5 groups(10 mice in each group).Each group was treated in a different way. The volume of the tumors were measured weekly for 8 weeks, then the growth curves of transplanted tumors were draw. Eight weeks later MVD was measured. Results Compared with control and bFGF group,The growth of tumor in each bFGF-MAb group was slow and the inhibitory effect of tumor was significant（P0.01）; The MVD expression were all decreased（P0.01）. The inhibitory effect of 30μg/ml bFGF-M group on tumor growth and angiogenesis was the most significant in all groups. Conclusion bFGF-Mab agent has good therapeutic effect in the treatment of nude transplanted human invasive pituitary adenoma.The mechanism o