amiloride enhances antigen specific ctl by faciliting hbv dna vaccine entry into cells阿米洛利增强抗原特异性ctl faciliting hbv dna疫苗进入细胞.pdfVIP

Amiloride Enhances Antigen Specific CTL by Faciliting HBV DNA Vaccine Entry into Cells 1 2 1 1 1 1 1 Shuang Geng , Yiwei Zhong , Shuang Wang , Hu Liu , Qiang Zou , Xiaoping Xie , Chaofan Li , Qingling 3 3 1,2 Yu , Zhonghuai He , Bin Wang * 1 State Key Laboratory for Agro-Biotechnology, China Agricultural University, Beijing, China, 2 Key laboratory of Medical Molecular Virology of MOH and MOE, Shanghai Medical College, Fudan University, Shanghai, China, 3 Beijing Advaccine Biotechnology, Beijing, China Abstract The induction of relatively weak immunity by DNA vaccines in humans can be largely attributed to the low efficiency of transduction of somatic cells. Although formulation with liposomes has been shown to enhance DNA transduction of cultured cells, little, if any, effect is observed on the transduction of somatic tissues and cells. To improve the rate of transduction, DNA vaccine delivery by gene gun and the recently developed electroporation techniques have been employed. We report here that to circumvent requirement for such equipment, amiloride, a drug that is prescribed for hypertension treatment, can accelerate plasmid entry into antigen presenting cells (APCs) both in vitro and in vivo. The combination induced APCs more dramatically in both maturation and cytokine secretion. Amiloride enhanced development of full CD8 cytolytic function including induction of high levels of antigen specific CTL and expression of IFN- c+perforin+granzymeB+ in CD