有哪些信誉好的足球投注网站aid activity in b cells strongly correlates with polyclonal antibody affinity maturation in-vivo following pandemic 2009-h1n1 vaccination in humans援助活动b细胞强烈与多克隆抗体亲和力成熟已2009年大流行h1n1疫苗接种后人类.pdfVIP
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AID Activity in B Cells Strongly Correlates with Polyclonal
Antibody Affinity Maturation in-vivo Following
Pandemic 2009-H1N1 Vaccination in Humans
1 2 2 1
Surender Khurana *, Daniela Frasca , Bonnie Blomberg , Hana Golding *
1 Division of Viral Products, Center for Biologics Evaluation and Research (CBER), Food and Drug Administration (FDA), Bethesda, Maryland, United States of America,
2 Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, Florida, United States of America
Abstract
The role of Activation-Induced Cytidine Deaminase (AID) in somatic hypermutation and polyclonal antibody affinity
maturation has not been shown for polyclonal responses in humans. We investigated whether AID induction in human B
cells following H1N1pdm09 vaccination correlated with in-vivo antibody affinity maturation against hemagglutinin domains
in plasma of young and elderly individuals. AID was measured by qPCR in B cells from individuals of different ages
immunized with the H1N1pdm09 influenza vaccine. Polyclonal antibody affinity in human plasma for the HA1 and HA2
domains of the H1N1pdm09 hemagglutinin was measured by antibody-antigen complex dissociation rates using real time
kinetics in Surface Plasmon Resonance. Results show an age-related decrease in AID induction in B cells following
H1N1pdm09 vaccination. Levels of AID mRNA before vaccination and fold-increase of AID mRNA expression after
H1N1pdm09 vaccination directly correlated with increase in polyclonal antibody affinity to the HA1 globular domain (but
not to the conserved HA2 stalk). In the younger population, significant affinity maturation to the HA1 g
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