ago hits-clip expands understanding of kaposis sarcoma-associated herpesvirus mirna function in primary effusion lymphomas前hits-clip扩大理解卡波济氏肉瘤相关疱疹病毒microrna的函数在初级积液淋巴瘤.pdfVIP

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ago hits-clip expands understanding of kaposis sarcoma-associated herpesvirus mirna function in primary effusion lymphomas前hits-clip扩大理解卡波济氏肉瘤相关疱疹病毒microrna的函数在初级积液淋巴瘤.pdf

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ago hits-clip expands understanding of kaposis sarcoma-associated herpesvirus mirna function in primary effusion lymphomas前hits-clip扩大理解卡波济氏肉瘤相关疱疹病毒microrna的函数在初级积液淋巴瘤

Ago HITS-CLIP Expands Understanding of Kaposi’s Sarcoma-associated Herpesvirus miRNA Function in Primary Effusion Lymphomas 1 1 1 1 1 1,2 Irina Haecker , Lauren A. Gay , Yajie Yang , Jianhong Hu , Alison M. Morse , Lauren M. McIntyre , Rolf Renne1,2,3* 1 Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, Florida, United States of America, 2 UF Genetics Institute, University of Florida, Gainesville, Florida, United States of America, 3 UF Shands Cancer Center, University of Florida, Gainesville, Florida, United States of America Abstract KSHV is the etiological agent of Kaposi’s sarcoma (KS), primary effusion lymphoma (PEL), and a subset of multicentricCastleman’s disease (MCD). The fact that KSHV-encoded miRNAs are readily detectable in all KSHV-associated tumors suggests a potential role in viral pathogenesis and tumorigenesis. MiRNA-mediated regulation of gene expression is a complex network with each miRNA having many potential targets, and to date only few KSHV miRNA targets have been experimentally determined. A detailed understanding of KSHV miRNA functions requires high-through putribonomics to globally analyze putative miRNA targets in a cell type-specific manner. We performed Ago HITS-CLIP to identify viral and cellular miRNAs and their cognate targets in two latently KSHV-infected PEL cell lines. Ago HITS-CLIP recovered 1170 and 950 cellular KSHVmiRNA targets from BCBL-1 and BC-3, respectively. Importantly, enriched clusters contained KSHV miRNA seed matches in the 39UTRs of numerous well characterized targets, among them THBS1, BACH1, and C/EBPb. KSHV miRNA targets were strongly enriched for genes involved in mu