a novel strategy to screen bacillus calmette-guérin protein antigen recognized by γδ tcr一项新策略来屏幕卡介苗蛋白质抗原被γδ识别.pdfVIP

´ A Novel Strategy to Screen Bacillus Calmette-Guerin Protein Antigen Recognized by cd TCR 1 1 1 1 1 2 3 3 XueYan Xi , XiaoYan Zhang , Bei Wang , Ji Wang , He Huang , LianXian Cui , XiQin Han , Liang Li , Wei 2 1 He *, ZhenDong Zhao * 1 State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China, 2 Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China, 3 Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, China Abstract Background: Phosphoantigen was originally identified as the main cd TCR-recognized antigen that could activate cd T cells to promote immune protection against mycobacterial infection. However, new evidence shows that the cd T cells activated by phosphoantigen can only provide partial immune protection against mycobacterial infection. In contrast, whole lysates of Mycobacterium could activate immune protection more potently, implying that other cd TCR-recognized antigens that elicit protective immune responses. To date, only a few distinct mycobacterial antigens recognized by the cd TCR have been characterized. Methodology/Principal Findings: In the present study, we established a new approach to screen epitopes or protein ´ antigens recognized by the cd TCR using Bacillus Ca