有哪些信誉好的足球投注网站a gold nanoparticle and aflatoxin b1-bsa conjugates based lateral flow assay method for the analysis of aflatoxin b1基于金纳米颗粒和黄曲霉毒素b1-bsa配合侧流试验方法分析黄曲霉毒素b1.pdfVIP
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a gold nanoparticle and aflatoxin b1-bsa conjugates based lateral flow assay method for the analysis of aflatoxin b1基于金纳米颗粒和黄曲霉毒素b1-bsa配合侧流试验方法分析黄曲霉毒素b1
Materials 2012, 5, 634-643; doi:10.3390/ma5040634
OPEN ACCESS
materials
ISSN 1996-1944
/journal/materials
Article
A Gold Nanoparticle and Aflatoxin B1-BSA Conjugates Based
Lateral Flow Assay Method for the Analysis of Aflatoxin B1
Jihea Moon, Giyoung Kim * and Sangdae Lee
Post-Harvest Food Engineering Division, Department of Agricultural Engineering, National
Academy of Agricultural Sciences, Suwon 441-707, Korea; E-Mails: mmir95@ (J.M.);
sdlee96@ (S.L.)
* Author to whom correspondence should be addressed; E-Mail: giyoung@korea.kr;
Tel.: +82-31-290-1899; Fax: +82-31-290-1900.
Received: 21 February 2012; in revised form: 28 March 2012 / Accepted: 5 April 2012 /
Published: 13 April 2012
Abstract: A rapid and simple immuno-chromatographic assay was developed to detect
aflatoxin B1 (AFB1). The assay was based on a modified competitive binding format using
colloidal gold and polyclonal antibody (Pab) conjugates. The anti-AFB1 Pab was
immobilized to a defined detection zone on a porous nitrocellulose membrane and colloidal
gold particles were conjugated to AFB1-BSA which served as a detection reagent. The
AFB1-containing sample was added to the membrane and allowed to move with
AFB1-BSA-coated particles dried on the conjugation pad. The mixture was then passed
along the porous membrane by capillary action past the Pab in the detection zone, which
captured AFB1 or AFB1-BSA. AFB1 in the sample inhibits
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