a complete developmental sequence of a drosophila neuronal lineage as revealed by twin-spot marcm果蝇神经细胞谱系的一个完整的发展序列揭示了twin-spot marcm.pdfVIP

A Complete Developmental Sequence of a Drosophila Neuronal Lineage as Revealed by Twin-Spot MARCM 1. 2. 2 2 2 1,2 Hung-Hsiang Yu , Chih-Fei Kao , Yisheng He , Peng Ding , Jui-Chun Kao , Tzumin Lee * 1 Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, United States of America, 2 Department of Neurobiology, University of Massachusetts, Worcester, Massachusetts, United States of America Abstract Drosophila brains contain numerous neurons that form complex circuits. These neurons are derived in stereotyped patterns from a fixed number of progenitors, called neuroblasts, and identifying individual neurons made by a neuroblast facilitates the reconstruction of neural circuits. An improved MARCM (mosaic analysis with a repressible cell marker) technique, called twin-spot MARCM, allows one to label the sister clones derived from a common progenitor simultaneously in different colors. It enables identification of every single neuron in an extended neuronal lineage based on the order of neuron birth. Here we report the first example, to our knowledge, of complete lineage analysis among neurons derived from a common neuroblast that relay olfactory information from the antennal lobe (AL) to higher brain centers. By identifying the sequentially derived neurons, we found that the neuroblast serially makes 40 types of AL projection neurons (PNs). During embryogenesis, one PN with multi-glomerular innervation and 18 uniglomerular PNs targeting 17 glomeruli of the adult AL are born. Many more PNs of 22 additional types, including four types of polyglomerular PNs, derive after the neuroblast resumes dividing in early lar