Nm23-H1在小细胞肺癌中的表达及其与预后的关系.docVIP

Nm23-H1在小细胞肺癌中的表达及其与预后的关系 熊艳丽，杨雪琴，张诗珩，杨宇馨，冯燕，黄环，王东(400042重庆，第三军医大学大坪医院野战外科研究所肿瘤中心) [摘要] 目的 探讨肿瘤转移抑制因子Nm23-H1在小细胞肺癌（SCLC）中的表达及其与预后的关系。方法 通过免疫组化SP法检测71例SCLC组织和22例正常肺组织中Nm23-H1的表达情况，根据染色强度和阳性肿瘤细胞数目所占百分比，将其分为高、低表达两组，使用SPSS软件统计分析Nm23-H1的表达与临床病理及预后的关系。结果 Nm23-H1蛋白表达在正常肺组织显著高于SCLC组织（90.9% vs. 57.7%，P=0.004）；局限期患者Nm23-H1蛋白表达显著高于广泛期患者（66.7% vs. 39.1%，P=0.028）。多因素Logistic回归分析显示，Nm23-H1蛋白可作为SCLC是否转移的独立保护因素（OR=0.32，95% CI=0.12-0.90，P=0.031）。亚细胞定位在正常肺组织与肺癌组织中没有差异，在不同分期之间也没有差异。多因素COX风险回归分析表明只有临床分期为广泛期和年龄≥60是SCLC患者总生存的劣势预后因素（年龄：HR=1.03，95% CI=1.00-1.05，P=0.029；临床分期：HR=1.85，95% CI=1.03-3.33，P=0.041）。Nm23-H1表达及亚细胞定位与SCLC患者生存期都没有显著相关性。结论 Nm23-H1在SCLC组织中以胞浆表达为主的低表达，低表达与SCLC的远处转移密切相关，可能是SCLC预后不良的指标之一。 [关键词] 小细胞肺癌；转移；Nm23-H1；预后 [中图法分类号] R734.1 [文献标志码] A The expression of Nm23-H1 gene product and its relationship with prognosis in patients with small cell lung cancer Xiong Yanli, Yang Xueqin, Zhang Shiheng, Yang Yuxin, Feng Yan, Huang Huan, Wang Dong（Cancer Center, Daping Hospital and Research Institution of Surgery, Third Military Medical University, Chongqing 400042 China） [Abstract] Objective To study the expression of Nm23-H1 gene product and its relationship with prognosis in patients with small cell lung cancer. Methods The expression of Nm23-H1 was detected by immunohistochemistry in 71 small cell lung cancer tissue samples and 22 normal lung tissue samples. According to their positive percentage and staining intensity, they were divided into two groups：high expression group and low expression group. The correlation of Nm23-H1 expression with clinicopathology and prognosis was performed by SPSS 19.0. Results The expression level of Nm23-H1 was higher in normal tissue samples than in small cell lung cancer（90.9% vs. 57.7%，P=0.004）；It was higher in limited stage than in extensive stage small cell lung cancer（66.7% vs. 39.1%，P=0.028）。 Multivariate logistic regression showed the Nm23-H1 expression was an independent protect factor for the metastasis of s