基于18srrna和线粒体16srrna基因序列的柳蚕-应用昆虫学报.pdfVIP

昆虫知识 Chinese Bulletin of Entomology 20 10 ，47 (2 ):285 ～ 292 基于18S rRNA 和线粒体16S rRNA 基因序列的 柳蚕进化分析*  朱保建 刘朝良 曹 甲 李旭全 魏国清 ( 230036 ) 安徽农业大学生命科学学院 合肥 Phylogenetic analysis of A ctias selene based on 18S ribosomal RNA and mitochondrial 16 S rRNA genes.  ZHU Bao-Jian ，LIU Chao-Liang ，CAO Jia ，LI Xu-Quan ，WEI Guo-Qing (College of Lif e Sciences ，A nhui Agriculture University ，Hefei 230036 ，China) Abstract To investigate the phylogenetic relationship between A ctias selene Hübner and other Lepidopteran insects ，a 39 1 bp fragment of 18S ribosomal RNA and a 428 bp fragment of mitochondrial 16 S rRNA were used to construct phylogenetic trees with neighbor-joining (NJ )，maximum parsimony (MP)and arithmetic averages (UPGMA )methods. The results showed the nucleotide composition of mitochondrial 16 S rRNA gene of A . selene had AT bias ，similar in other Saturnidae insects. The genetic distances between A . selene and other species ranged from 0. 0 16 to 0. 140 based on mitochondrial 16 S rRNA gene sequences ，in which the minimum distance was that to A ntheraea roylii and the maximum was that to Bombyx mandarina. As far as 18S rRNA gene sequences were concerned ，the minimum distance (0 . 0 10 )and the maximum distance (0 . 0 17 )were showed between A . selene with A . p ernyi ，and Samia ricini ，respectively . Key words A ctias selene ，18S ribosomal RNA ，mitochondrial 16 S rRNA ，phylogenetic relationship A ctias selene Hübner ， PCR 摘 要 为探讨柳蚕 与鳞翅目昆虫的系统发育关系 本研究利用 扩增获得了