系统性红斑狼疮与il-12（Systemic lupus erythematosus and IL-12）.docVIP

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系统性红斑狼疮与il-12（Systemic lupus erythematosus and IL-12）.doc

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系统性红斑狼疮与il-12（Systemic lupus erythematosus and IL-12）

系统性红斑狼疮与il-12（Systemic lupus erythematosus and IL-12） Systemic lupus erythematosus and IL-12 2010-04-23 Zhang Fengshan branch Tan Jinglan Wang Zhenyu Lu Bin Wu Changyou Ngoc Huong IL-12 natural killer cell stimulatory factor has immunomodulatory effect is very strong, it is known in Th1 cell mediated autoimmune diseases especially play a major role in organ specific autoimmune disease, and systemic autoimmune disease in the role is still not clear, the purpose of this study is to explore the relationship between IL-12 and SLE. In order to further reveal the pathogenesis of SLE, to explore the new treatment approach. 1 data and methods 1.1 cases: the study of 18 cases of SLE patients were severe patients (i.e. renal or central nervous system damage in patients), male 1 cases, female 17 cases, were consistent with the diagnostic criteria of American Rheumatism Association in 1982 SLE. The average age was 27.35 years. Methods 13 healthy workers were chosen as the control, including 2 males and 11 females, with an average age of 30.27 years. 1.2 experimental materials: all the IL-12 and IL-12P40 standards, kits, antigens, antibodies and other reagents used in this experiment were provided by the United States Hoffmann-La, Roche, Inc. 1.3 experimental method: the experimental operation methods were provided by American Hoffmann-La, Roche and Inc. The details are as follows: the subjects were collected 5 ml peripheral blood, anticoagulation, plus liquid layer separating lymphocytes cells suspended in culture medium containing AB serum, cultured in 5% CO2 incubator, each cell two, a plus lipopolysaccharide (LPS) stimulation, a plus. After 48 hours culture, supernatant was taken. The concentration of IL-12 and IL-12P40 was detected by ELISA double antibody sandwich method. All the results were expressed by + s, and the significance of the difference among the groups was detected by t test. 2 Result The levels of IL-12 and IL-12P40 in peripheral lymphocyte culture supernatan