病理切片染色（Pathological section staining）.docVIP

病理切片染色（Pathological section staining） This routine pathological examination notice. 1. the tissue specimens cut during operation should be fixed in fixed liquid immediately (operation room should be provided with specimen bottle and fixative). Usually 10% neutral marlin is used as fixative. The amount of fixative shall not be less than 5~10 times the volume of the specimen. The mouth of the specimen should be big enough to be removed after the specimen is fixed. The specimen is fixed to the bottle wall and the bottom of the bottle. The absorbent is made of absorbent cotton pad. The floating surface is covered with absorbent cotton. If it is an infectious specimen, it should be careful not to pollute the outside of the container. 2. specimen containers, should be affixed with the patients name or label for single chain. As a patient has several tissues at the same time, or the same tissue is taken out of different parts, different containers shall be packed and marked separately. Specimens of different patients should not be placed in the same container. Take 3. samples, pay attention to not use toothed forceps or clamp, do not squeeze, to avoid man-made deformation, affect the diagnosis. Dont open the specimen before delivery. Keep the prototype and submit it all. When it is necessary to open the case, it is better to invite the pathologist to the scene. Otherwise, the specimen should be described in detail before and after the pathological examination application. Check the organization should not be too small, so as not to affect the diagnosis. 4. specimens such as whole organ or department, or larger, is not fixed, but should send pathology treatment (treatment field or long specimen, can refer to section third for testing). 5. samples of various body fluids, punctures and cytology should be examined immediately. If not for inspection immediately, then the centrifugal precipitation will make uniform sediment smear from 2 to 3, in a timely manner into the fixed et