生物实验（Biological experiment）.docVIP

生物实验（Biological experiment） Summary of knowledge points of biology experiments in Senior High School In Experiment 1, the distribution of DNA and RNA in cells was observed Experiment principle: DNA green, RNA red Distribution: eukaryotic DNA is mainly distributed in the nucleus, and contains a small amount of DNA in mitochondria and chloroplasts. RNA is mainly distributed in the cytoplasm. The results showed that the nucleus was green and the cytoplasm was red Experiment two material identification Reducing sugar + Fehling reagent to brick red precipitate. Fat + Sultan III ~ orange Fat + Sultan IV to red Protein + biuret reagent to purple reaction 1. Determination of reducing sugar (1) material selection: reducing sugar content is high, white or nearly white, such as apples, pears, white radish. (2): Fehling Reagent (a liquid reagent: NaOH solution, 0.1g/mL solution B: 0.05g/mL CuSO4 solution), mix it with. (3) steps: sampling 2mL liquid adding Fehling reagent 1mL just matched in a test tube, (a liquid Fehling reagent and the second liquid mixed evenly and then join), about 2min, water bath heating observe the color change (white, light blue, red brick) Detection of urine sugar simulation. 1. Sampling: urine of normal subjects and urine of diabetic patients 2, detection methods: Fehling Reagent (water bath heating) or Bancroft reagent or dipstik Results: (3, by Fehling reagent detection) occurrence of brick red precipitate is diabetic urine test tube, no brick red precipitate is normal human urine. 4, analysis: because of containing reducing sugar in the urine of patients with diabetes, react to produce brick red precipitate with Fehling reagent, and normal human urine without reducing sugar, so no reaction occurred. 2, fat detection (1) material selection: the higher the fat content, the better the cotyledon. (2) step: make the slice (thinner, the better). Slice the thinnest peanuts in the center of the slide Here (Sultan III staining dye 2 ~ 3 drops of slice, 2 ~