现代试管婴儿选精技术（Selection technique of modern test tube baby）.docVIP

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2017-09-02 发布于河南
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现代试管婴儿选精技术（Selection technique of modern test tube baby）.doc

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现代试管婴儿选精技术（Selection technique of modern test tube baby）

现代试管婴儿选精技术（Selection technique of modern test tube baby） Sperm selection technology More boys and girls are looking at men, and sperm selection is a way of meeting peoples demands for sex. Male requirements can collect semen and semen in vitro. Generally speaking, semen is not less than 0.5ml at a time, the sperm density is not less than 10 million / ml, and the routine examination index of semen becomes more normal, and the higher the rate of fertilization success. Screening technique of sperm in vitro With the development of assisted reproductive technology, a variety of in vitro sperm screening techniques have emerged. At present, the clinical application of in vitro selection, sperm separation methods are various, mainly include: glass fiber filtration, sperm electrophoresis, sperm swimming method, serum albumin filtration method, Percol gradient separation and migration of precipitation (migration sedimentation). The technique of sperm selection in the five star reproductive center is the most advanced in China. Many selected papers on test tube babies have been published in many academic journals both at home and abroad. (I) sperm swimming separation technique; The principle is based on the movement of sperm to swim with liquid interface ability of liquid diffusion, which itself and dead sperm, sperm agglutination, abnormal sperm, white blood cells and impurities can be separated by electrophoresis on the high activity of sperm. Although the relationship between the migration ability of spermatozoa and their fertilization ability needs to be studied further, more and more clinical studies have proved that the effect is obvious in the past 10 years. 1. reagent (1) culture medium: modified F10 culture medium, Earle culture medium, B2 culture medium, etc. (see section on an introduction to several common formulas for culture fluids). (2) human serum albumin (HSA) or inactivated umbilical cord blood serum. When swimming, HSA can be added to the medium (1g / L) or