Isolation and screening of potential cellulolytic （分离和筛选潜在的纤维素分解）.pdfVIP

INT J CURR SCI 2013, 8: E 125-132 RESEARCH ARTICLE ISSN 2250-1770 Isolation and screening of potential cellulolytic fungi from Areca nut husk waste Naveenkumar KJ and Thippeswamy B* Department of PG Studies and Research in Microbiology, Bioscience Complex, Kuvempu University, Jnana Sahyadri Shankaraghatta-577 451, Shivamogga District, Karnataka, India *Corresponding author: thippeswamyb205@; Tel: +91-9986917039; Fax: +91-08282-256262 Abstract The present study was aimed to isolate and screen the ability of cellulolytic fungal strains from areca nut husk waste to produce cellulolytic enzymes for use in the bioconversion of waste products into valuable biomass products. Out of twenty eight fungal culture isolates from areca nut husk waste, twenty four fungi were able to degrade the cellulose. The highest cellulolytic activity was detected in only ten isolates viz. Aspergillus niger (16±0.6 mm), Aspergillus terreus (15±0.5 mm), Trichoderma viride (13±0.3 mm), Aspergillus flavus (12±0.3 mm), Fusarium chlamydosporum (10±0.04 mm), Aspergillus fumigatus (9.5±0.2 mm), Aspergillus clavatus (9.0±0.05 mm), Paecilomyces carneus (8.0±0.2 mm), Penicillium chrysogenum (7.2±0.3 mm) and Aspergillus wentii (7.0±0.2 mm) then compared to other fungi. While Aspergillus sp 2, Penicillium sp 3, Heteroconium chaetospira and Staphylotrichum coccosporums were not produce cellulase enzymes. These fungal strains are very important for degradation of agricultural wastes, municipal solid wastes and some other organic wastes. Keywords: screening, fungi, cellulolytic activity, areca nut husk waste th