Guidelines for Use and Care of Aminex Resin （Aminex树脂的使用指南和护理）.PDFVIP

Guidelines for Use and Care of Aminex® Resin-Based Columns Instruction Manual For technical service call your local Bio-Rad office or in the U.S. Call 1-800-4BIORAD (1-800-424-6723) Table of Contents Section 1 Introduction to Resin-Based HPLC Columns 1 Section 2 Column Set-Up 2 2.1 Unpacking 2 2.2 Preparing the Eluant 2 Section 3 Guard Columns 2 3.1 Installing Guard Columns 3 3.2 Purging the Guard Columns 3 Section 4 Attaching the Column 4 Section 5 Operating Parameters 5 5.1 Flow Rate 5 5.2 Sample Preparation 6 5.3 Pressure Checks 6 5.4 Column Testing7 Section 6 Regeneration Procedures 14 6.1 Fluffing the Resin Bed 14 6.2 Cleaning a Contaminated Column14 6.3 Reconverting a Column to its Original Ionic Form 15 6.4 Topping Off a Column Bed 15 6.5 Rectifying Instrument/Connecting Tubing Problems ..16 Section 7 Heated Column Shut-Down 16 Section 8 Column Storage17 8.1 Long Term Storage17 8.2 Short Term Storage 17 Section 9 Troubleshooting17 Section 1 Introduction to Resin-Based HPLC Columns Resin-based HPLC columns use the mechanisms of ion exclusion, ion exchange, ligand exchange, size exclusion, reversed phase, and normal phase partitioning. These multiple modes of interaction offer a unique ability to separate compounds. The charge on the resin provides the capability for ion exclusion, while the polystyrene backbone allows hydrophobic