Enzyme Linked Immunosorbent Asssay Enzyme （酶联免疫吸附Asssay酶）.pdfVIP

Enzyme Linked Immunosorbent Assay Enzyme Linked Immunosorbent Assay Enzyme Linked Immunosorbent Assay ELISA ELISA ELISA I I I Principles Principles Principles J. R. Crowther J. R. Crowther J. R. Crowther Joint FAO/IAEA Division Joint FAO/IAEA Division Joint FAO/IAEA Division Vienna Vienna Vienna SECTION ONE SECTION ONE What do we need to know to run a good ELISA? What do we need to know to run a good ELISA? Various disciplines in science are needed to allow optimal use of ELISA and these are outlined here. Attention to increasing knowledge in the areas highlighted is essential both in developmental work and in the ultimate value of any test devised. Defining, as early as possible, the OBJECTIVES FOR THE USE OF THE ELISA is essential. The operator has to have an understanding all other published work concerning the problem. Development of a diagnostic test for a specific disease requires that all other data pertaining to the biology of that disease, e.g., antigenicity and structure of the agent; antibody production in different animals following infection; qualitative assessment of antibodies by different assays and availability of standard or control sera, etc are examined. Attention to laboratory facilities is essential, e.g., equipment; reagents already developed; laboratory small animals; experimental large animals; cash to buy