NBQX对脑缺血再灌注后成年大鼠大脑少突胶质前体细胞的保护作用.docVIP

　　NBQX对脑缺血再灌注后成年大鼠大脑少突胶质前体细胞的保护作用 ：李华杰 吴 坚 盛世英 朱林凤 张菊美 【摘要】 目的 探讨脑缺血再灌注后成年大鼠大脑少突胶质前体细胞的变化及MK801和NBQX对其保护作用。方法 以线栓法制作成年SD大鼠局灶性脑缺血再灌注模型(阻塞90min再灌注1d、7d和14d)，分别向其大脑皮质缺血区立体定向注射5mmol/L 的MK801和50 mmol/L的NBQX各1μl，用免疫荧光组织化学法检测脑缺血再灌注后成年大鼠早期大脑梗死中心区、梗死周边区和缺血对侧NG2和O4阳性细胞数量。结果 脑缺血再灌注后成年大鼠大脑皮质梗死中心区NG2 和O4阳性细胞逐渐减少， NBQX组大鼠NG2和O4阳性细胞数减少的幅度较少。脑缺血再灌注后梗死周边区NG2和O4阳性细胞数逐渐增加，NBQX组大鼠增加更明显，而MK801组与生理盐水组无明显差别。结论 成年SD大鼠脑缺血再灌注后梗死周边区少突胶质前体细胞增多。NBQX对少突胶质前体细胞早期的缺血性损伤有保护作用。 【关键词】 缺血;再灌注;少突胶质前体细胞;NBQX 　　【Abstract】 Objective To explore the change of oligodendrocyte precursor cells in adult rats after focal cerebral ischemia and the protection of MK801 and NBQX to them. Methods Focal cerebral ischemia and reperfusion model in male adult SD rats iddle artery inutes and then reperfusion for 1 day, 7 days and 14 days.One microliter MK801 (5mmol/L) and NBQX (50mmol/L) ic area of SD rats under stereodirectional instrument right after ischemia.Then NG2,O4 positive cells in the ischemic core, ischemic penumbra and contralateral area of rats inated at each timepoint after reperfusion by immunofluorescence histochemistry. Results The number of NG2 , O4 positive cells ic core decreased significantly at each timepoint ount. The number of NG2 and O4positive cells ic penumbra increased gradually after reperfusion ount. Conclusion The marked increase of oligodendrocyte precursor cells is observed in the periinfarct area during the postischemic reperfusion period in the adult rat brain. And NBQX has some neuroprotective effect to oligodendrocyte precursor cells in postischemic brain injury. 　　【Key ia; Reperfusion; Oligodendrocyte precursor cells; NBQX 　　兴奋性氨基酸毒性损伤作用是脑创伤和脑卒中等中枢神经系统(central nervous system，S)疾病中神经元死亡的主要因素之一[1]。而少突胶质前体细胞(oligodendrocyte precursor cells, OPC)膜上含有大量的兴奋性氨基酸受体[2]。MK801和NBQX分别是N甲基D天冬氨酸(NmethylDaspartate，NMDA)受体和α氨基3羟基5甲基4异唑丙酸盐(alphaamino3hydroxy5methyl4isoxazole propionate，AMPA)受体的拮抗剂。我们观察了脑定向注射MK801和NBQX对大鼠脑缺血再灌注后早期大脑皮质NG2和O4表达的影响，报道如下。 　　1 材料和方法 　　1.