中国南瓜种质资源农艺性状与rapd标记分析-核农学报.pdfVIP

※工艺技术 食品科学 2010, Vol. 31, No. 22 237 鮰 杨晓军，陆剑锋 * ，林 琳，段瑞霞，翁世兵，叶应旺，姜绍通 (合肥工业大学生物与食品工程学院，安徽 合肥 230009) ：以斑点叉尾鮰内脏为原料，血管紧张素转化酶抑制率为指标，筛选出木瓜蛋白酶为酶解的最适蛋白酶， 并研究该酶的酶解时间、酶添加量、初始pH 值、酶解温度、液料比(mL/g)对酶解产物抑制活性的影响，通过正 交试验优化得到了具有 ACE 抑制活性的酶解产物的最佳工艺条件。结果表明：最优酶解条件为初始 pH7.5 、酶解 温度 55 ℃、液料比2:1(mL/g) ，酶解产物ACE 抑制率为 72.34% 。 ：斑点叉尾鮰；鱼内脏；抑制率；血管紧张素转化酶；木瓜蛋白酶 Enzymatic Preparation of Angiotensin Ⅰ-converting Enzyme (ACE) Inhibitory Products Derived from Viscera of Channel Catfish YANG Xiao-jun ，LU Jian-feng* ，LIN Lin ，DUAN Rui-xia ，WENG Shi-bing ，YE Ying-wang ，JIANG Shao-tong (School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China) Abstract ：In this study, viscera of channel catfish, Ictalurus punctatus, were used as the raw materials to prepare angiotensin Ⅰ-converting enzyme (ACE) inhibitory products on the basis of its inhibitory rate of ACE. Papain was identified as the optimal enzyme. The effects of enzymatic hydrolysis time, enzyme addition amount, initial pH, hydrolysis temperature, and liquid- material ratio on the inhibitory rate of prepared ACE inhibitory products were investigated by orthogonal experiments. Results indicated that the optimal enzymatic hydrolysis conditions were initial pH of 7.5, hydrolysis temperature of 60 ℃, and liquid- material ratio of 2:1. Under the optimal conditions, the ACE inhibitory rate of enzymatic hydrolysate reached up to 72.34%. Key words ：Ictalurus punctatus ；fish viscera ；inhibitory rate ；angiotensin Ⅰ-converting enzyme ；papain 中图分类号：TS254.9 文献标识码：A 文章编号：1002-6630(2010)22-0237-05 在中国，高血压患病人数多达 1.2 亿，每年死于心 水产品加工业也逐渐发展起来，但大部分加工企业只对 血管疾病的人数达