identification and in vivo characterization of nvfp-7r, a developmentally regulated red fluorescent protein of nematostella vectensisnvfp-7r体内识别和特征,发育调节的红色荧光蛋白nematostella vectensis.pdfVIP

Identification and In Vivo Characterization of NvFP-7R, a Developmentally Regulated Red Fluorescent Protein of Nematostella vectensis 1 1,2 Aissam Ikmi , Matthew C. Gibson * 1 Stowers Institute for Medical Research, Kansas City, Missouri, United States of America, 2 Department of Anatomy and Cell Biology, Kansas University Medical School, Kansas City, Kansas, United States of America Abstract Background: In recent years, the sea anemone Nematostella vectensis has emerged as a critical model organism for comparative genomics and developmental biology. Although Nematostella is a member of the anthozoan cnidarians (known for producing an abundance of diverse fluorescent proteins (FPs)), endogenous patterns of Nematostella fluorescence have not been described and putative FPs encoded by the genome have not been characterized. Methodology/Principal Findings: We described the spatiotemporal expression of endogenous red fluorescence during Nematostella development. Spatially, there are two patterns of red fluorescence, both restricted to the oral endoderm in developing polyps. One pattern is found in long fluorescent domains associated with the eight mesenteries and the other is found in short fluorescent domains situated between tentacles. Temporally, the long domains appear simultaneously at the 12-tentacle stage. In contrast, the short domains arise progressively between the 12- and 16-tentacle stage. To determine the source of the red fluorescence, we used bioinformatic approaches to identify all possible putative Nematostella FPs and a Drosophila S2 cell culture assay to validate NvFP-7R, a novel red fluorescent protein. We report that both the mRNA expression pa