icaritin shows potent anti-leukemia activity on chronic myeloid leukemia in vitro and in vivo by regulating mapkerkjnk and jak2stat3 akt signalingsicaritin显示强力的抗白血病活性在慢性粒细胞白血病体外和体内通过调节mapkerkjnk jak2stat3 akt信号.pdfVIP

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icaritin shows potent anti-leukemia activity on chronic myeloid leukemia in vitro and in vivo by regulating mapkerkjnk and jak2stat3 akt signalingsicaritin显示强力的抗白血病活性在慢性粒细胞白血病体外和体内通过调节mapkerkjnk jak2stat3 akt信号.pdf

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icaritin shows potent anti-leukemia activity on chronic myeloid leukemia in vitro and in vivo by regulating mapkerkjnk and jak2stat3 akt signalingsicaritin显示强力的抗白血病活性在慢性粒细胞白血病体外和体内通过调节mapkerkjnk jak2stat3 akt信号

Icaritin Shows Potent Anti-Leukemia Activity on Chronic Myeloid Leukemia In Vitro and In Vivo by Regulating MAPK/ERK/JNK and JAK2/STAT3 /AKT Signalings 1. 1. 1 2 1 2 1 Jian feng Zhu , Zi jian Li , Guang sen Zhang *, Kun Meng *, Wen yong Kuang , Jin Li , Xin fu Zhou , 1 1 1 1 1 1 1 Rui juan Li , Hong ling Peng , Chong wen Dai , Jian Kai Shen , Fan jie Gong , Yun xiao Xu , Su fang Liu 1 Division of Hematology, Institute of Molecular Hematology, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, People’s Republic of China, 2 Shenogen Biomedical Co, Ltd, Beijing, Haidian, Beijing, People’s Republic of China Abstract Purpose: To explore the effects of Icaritin on chronic myeloid leukemia (CML) cells and underlying mechanisms. Method: CML cells were incubated with various concentration of Icaritin for 48 hours, the cell proliferation was analyzed by MTT and the apoptosis was assessed with Annexin V and Hoechst 33258 staining. Cell hemoglobinization was determined. Western blotting was used to evaluate the expressions of MAPK/ERK/JNK signal pathway and Jak-2/Phorpho-Stat3/Phorsph- Akt network-related protein. NOD-SCID nude mice were applied to demonstrate the anti-leukemia effect of Icaritin in vivo. Results: Icaritin potently inhibited proliferation of K562 cells (IC50 was 8 mM) and primary CML cells (IC50 was 13.4 mM for CML-CP and 18 mM for CML-BC), induced CML cells apoptosis and promoted the erythroid differentiation of K562 cells with time-dependent manner. Furthermore, Icaritin was able to suppress the growth of primary CD34+ leukemia cells (CML) and