genomic content of bordetella pertussis clinical isolates circulating in areas of intensive children vaccination百日咳博德特氏菌临床分离株的基因组内容循环密集地区的儿童接种疫苗.pdfVIP

Genomic Content of Bordetella pertussis Clinical Isolates Circulating in Areas of Intensive Children Vaccination ´ 1 ´ 1 2 3 1 Valerie Bouchez , Valerie Caro , Erwan Levillain , Ghislaine Guigon , Nicole Guiso * ´ ´ ´ ´ 1 Institut Pasteur, Unite Prevention et Therapie Moleculaires des Maladies Humaines, URA-CNRS 3012, Paris, France, 2 Institut Pasteur de Lille, Laboratoire d’Etudes ´ ´ ´ Transcriptomiques et Genomiques Appliquees-Plateforme Biopuces Lille, UMR 8161-IFR 142, Lille, France, 3 Institut Pasteur, Plateforme Sante Publique, Paris, France Abstract Background: The objective of the study was to analyse the evolution of Bordetella pertussis population and the influence of herd immunity in different areas of the world where newborns and infants are highly vaccinated. Methodology: The analysis was performed using DNA microarray on 15 isolates, PCR on 111 isolates as well as GS-FLX sequencing technology on 3 isolates and the B. pertussis reference strain, Tohama I. Principal Findings: Our analyses demonstrate that the current circulating isolates are continuing to lose genetic material as compared to isolates circulating during the pre-vaccine era whatever the area of the world considered. The lost genetic material does not seem to be important for virulence. Our study confirms that the use of whole cell vaccines has led to the control of isolates that were similar to vaccine strains. GS-FLX sequencing technology shows that current isolates did not acquire any additional material when compared with vaccine strains or with isolates of the pre-va