有哪些信誉好的足球投注网站genome-wide identification of small rnas in the opportunistic pathogen enterococcus faecalis v583全基因组的小分子rna识别机会病原体粪肠球菌v583.pdfVIP
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genome-wide identification of small rnas in the opportunistic pathogen enterococcus faecalis v583全基因组的小分子rna识别机会病原体粪肠球菌v583
Genome-Wide Identification of Small RNAs in the Opportunistic Pathogen Enterococcus faecalis V583 1 1 2 2 1 ´ Kouki Shioya , Charlotte Michaux , Carsten Kuenne , Torsten Hain , Nicolas Verneuil , Aurelie 1 3 1 1 Budin-Verneuil , Thomas Hartsch , Axel Hartke , Jean-Christophe Giard * 1 Laboratoire de Microbiologie de l’Environnement, EA956-USC INRA 2017-IFR146 ICORE, University of Caen, Caen, France, 2 Institute of Medical Microbiology, Justus- Liebig-University, Giessen, Germany, 3 Genedata AG, Basel, Switzerland Abstract Small RNA molecules (sRNAs) are key mediators of virulence and stress inducible gene expressions in some pathogens. In this work we identify sRNAs in the Gram positive opportunistic pathogen Enterococcus faecalis. We characterized 11 sRNAs by tiling microarray analysis, 59 and 39 RACE-PCR, and Northern blot analysis. Six sRNAs were specifically expressed at exponential phase, two sRNAs were observed at stationary phase, and three were detected during both phases. Searches of putative functions revealed that three of them (EFA0080_EFA0081 and EFB0062_EFB0063 on pTF1 and pTF2 plasmids, respectively, and EF0408_EF04092 located on the chromosome) are similar to antisense RNA involved in plasmid addiction modules. Moreover, EF1097_EF1098 shares strong homologies with tmRNA (bi-functional RNA acting as both a tRNA and an mRNA) and EF2205_EF2206 appears homologous to 4.5S RNA member of the Signal Recognition Particle (SRP) ribonucleoprotein complex. In addition, proteomic analysis of the DEF3314_EF3315 sRNA mutant suggests that it may be involved in t
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