基于aflp方法对不同地理种群棉褐带卷蛾分化-应用昆虫学报.docVIP

基于AFLP方法对不同地理种群棉褐带卷蛾遗传分化的研究 魏晓棠* ，，，，， (山东出入境检验检疫局，青岛 266002) 摘要：利用3对AFLP引物对我国棉褐带卷蛾4个地理种群进行了遗传共扩增得到224个DNA条带，其中多态条带195个均具有较高的遗传多样性水平Nei遗传多样性指数为0.1425~0.1578，Shannon多样性指数为0.2137~0.2383群体间的遗传分化系Gst）和基因流AMOVA分析结果66.81%的变异来源于种群内Adoxophyes orana based on AFLP WEI xiao-tang , ZHANG cheng-biao, ZHANG jing-xuan, NI xiu-mei, HU dong-qing, SONG tao ( Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao, Shandong 26600 ) Abstract: The AFLP technique was used to analyze the genetic differentiation among 4 populations of Adoxophyes orana by using 3 pairs of primers, and 224 DNA fragments were generated while 195 of 224 DNA fragments were polymorphic. The genetic diversity analysis of the populations showed high level of genetic variation among 4 populations, and the Shannon’s genetic diversity indexes varied from 0.2137 to 0.2383. Nei genetic diversity indexes were from 0.1425 to 0.1578. Remarkable genetic differentiation indicated by genetic differentiation coefficient (Gst) and gene flow (Nm) analysis among 4 populations, was detected between the Summer fruit tortrix populations in the North and the Smaller tea tortrix populations in the South. There was a relatively strong level of gene flow and a low extent of genetic differentiation among the three Summer fruit tortrix populations in the North. The result of AMOVA analysis indicated that a high percentage of the total genetic variance (66.81%) accounted for the variations among populations. The UPGMA cluster analysis indicated that the genetic distances were closer among Shandong, Beijing and Shanxi populations, and thus the three populations were categorized into one clade, while Fujian population should be categorized into another clade. The results suggested that the genetic distance might be correlated with their host comparability but not related with their geographic distance. Key words: Adoxophyes orana; AFLP; genetic differentiation; geographic populations 棉褐带卷蛾Adoxophyes orana (Fischer von Rosslerstamm