a topological map of the compartmentalized arabidopsis thaliana leaf metabolome拓扑地图的区分拟南芥叶代谢物.pdfVIP

A Topological Map of the Compartmentalized Arabidopsis thaliana Leaf Metabolome 1. 2. 2 2 ¨ 3 Stephan Krueger , Patrick Giavalisco , Leonard Krall , Marie-Caroline Steinhauser , Dirk Bussis , 2 ¨ 1 2 2 2 . Bjoern Usadel , Ulf-Ingo Flugge , Alisdair R. Fernie , Lothar Willmitzer , Dirk Steinhauser * 1 Botanical Institute, University of Cologne, Cologne, Germany, 2 Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany, 3 GABI Managing Office, c/o Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany Abstract Background: The extensive subcellular compartmentalization of metabolites and metabolism in eukaryotic cells is widely acknowledged and represents a key factor of metabolic activity and functionality. In striking contrast, the knowledge of actual compartmental distribution of metabolites from experimental studies is surprisingly low. However, a precise knowledge of, possibly all, metabolites and their subcellular distributions remains a key prerequisite for the understanding of any cellular function. Methodology/Principal Findings: Here we describe results for the subcellular distribution of 1,117 polar and 2,804 lipophilic mass spectrometric features associated to known and unknown compounds from leaves of the model plant Arabidopsis thaliana. Using an optimized non-aqueous fractionation protocol in conjunction with GC/MS- and LC/MS-based metabolite profiling, 81.5% of the metabolic data could be associated to one of three subcellular compartments: th