a systems approach reveals regulatory circuitry for arabidopsis trichome initiation by the gl3 and gl1 selectors监管电路系统方法揭示了拟南芥毛状体的起始gl3 gl1选择器.pdfVIP

A Systems Approach Reveals Regulatory Circuitry for Arabidopsis Trichome Initiation by the GL3 and GL1 Selectors Kengo Morohashi1,2, Erich Grotewold1,2,3* 1 Department of Plant, Cellular, and Molecular Biology, Ohio State University, Columbus, Ohio, United States of America, 2 Plant Biotechnology Center, Ohio State University, Columbus, Ohio, United States of America, 3 Mathematical Bioscience Institute, Ohio State University, Columbus, Ohio, United States of America Abstract Position-dependent cell fate determination and pattern formation are unique aspects of the development of plant structures. The establishment of single-celled leaf hairs (trichomes) from pluripotent epidermal (protodermal) cells in Arabidopsis provides a powerful system to determine the gene regulatory networks involved in cell fate determination. To obtain a holistic view of the regulatory events associated with the differentiation of Arabidopsis epidermal cells into trichomes, we combined expression and genome-wide location analyses (ChIP-chip) on the trichome developmental selectors GLABRA3 (GL3) and GLABRA1 (GL1), encoding basic helix-loop-helix (bHLH) and MYB transcription factors, respectively. Meta-analysis was used to integrate genome-wide expression results contrasting wild type and gl3 or gl1 mutants with changes in gene expression over time using inducible versions of GL3 and GL1. This resulted in the identification of a minimal set of genes associated with the differentiation of epidermal cells into trichomes. ChIP-chip experiments, complemented by the targeted examination of factors known to participate in trichome initiati