有哪些信誉好的足球投注网站a simple detection method for low-affinity membrane protein interactions by baculoviral display一个简单的检测方法对于低亲和力baculoviral显示膜蛋白相互作用.pdfVIP
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a simple detection method for low-affinity membrane protein interactions by baculoviral display一个简单的检测方法对于低亲和力baculoviral显示膜蛋白相互作用
A Simple Detection Method for Low-Affinity Membrane Protein Interactions by Baculoviral Display 1,2 1,2 1,2 3 4 Toshiko Sakihama *, Takato Sato , Hiroko Iwanari , Toshio Kitamura , Shimon Sakaguchi , Tatsuhiko Kodama2, Takao Hamakubo1,2 1 Department of Molecular Biology and Medicine, The University of Tokyo, Tokyo, Japan, 2 Laboratory for Systems Biology and Medicine, Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo, Japan, 3 Division of Cellular Therapy, Institute of Medical Science, The University of Tokyo, Tokyo, Japan, 4 Department of Experimental Pathology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan Abstract Background: Membrane protein interactions play an important role in cell-to-cell recognition in various biological activities such as in the immune or neural system. Nevertheless, there has remained the major obstacle of expression of the membrane proteins in their active form. Recently, we and other investigators found that functional membrane proteins express on baculovirus particles (budded virus, BV). In this study, we applied this BV display system to detect interaction between membrane proteins important for cell-to-cell interaction in immune system. Methodology/Principal Findings: We infected Sf9 cells with recombinant baculovirus encoding the T cell membrane protein CD2 or its ligand CD58 and recovered the BV. We detected specific interaction between CD2-displaying BV and CD58-displaying BV by an enzyme-linked immunosorbent assay (ELISA). Using this system, we also detected specific interaction between two other membrane receptor-ligand pairs, CD40-CD40
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