有哪些信誉好的足球投注网站a reverse transcriptase-pcr assay for detecting filarial infective larvae in mosquitoes反向transcriptase-pcr化验检测丝虫的感染蚊子的幼虫.pdfVIP
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a reverse transcriptase-pcr assay for detecting filarial infective larvae in mosquitoes反向transcriptase-pcr化验检测丝虫的感染蚊子的幼虫
A Reverse Transcriptase-PCR Assay for Detecting Filarial
Infective Larvae in Mosquitoes
1 1 1,2 1 3 4
Sandra J. Laney *, Caitlin J. Buttaro , Sabato Visconti , Nils Pilotte , Reda M. R. Ramzy , Gary J. Weil ,
Steven A. Williams1,5
1 Department of Biological Sciences, Smith College, Northampton, Massachusetts, United States of America, 2 Amherst College, Amherst, Massachusetts, United States of
America, 3 National Nutrition Institute, Cairo, Egypt, 4 Infectious Disease Division, Department of Internal Medicine, Washington University School of Medicine, St. Louis,
Missouri, United States of America, 5 Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, Massachusetts, United States of America
Abstract
Background: Existing molecular assays for filarial parasite DNA in mosquitoes cannot distinguish between infected
mosquitoes that contain any stage of the parasite and infective mosquitoes that harbor third stage larvae (L3) capable of
establishing new infections in humans. We now report development of a molecular L3-detection assay for Brugia malayi in
vectors based on RT-PCR detection of an L3-activated gene transcript.
Methodology/Principal Findings: Candidate genes identified by bioinformatics analysis of EST datasets across the B. malayi
life cycle were initially screened by PCR using cDNA libraries as templates. Stage-specificity was confirmed using RNA
isolated from infected mosquitoes. Mosquitoes were collected daily for 14 days after feeding on microfilaremic cat blood.
RT-PCR was performed with primer sets that were specific for individual candidate genes. Many promising candidates with
strong expression in the L3 stage were exc
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