a protein-protein interaction map of the trypanosoma brucei paraflagellar rod锥虫属的蛋白质交互地图brucei paraflagellar杆.pdfVIP

A Protein-Protein Interaction Map of the Trypanosoma brucei Paraflagellar Rod Sylvain Lacomble., Neil Portman., Keith Gull* Sir William Dunn School of Pathology and Oxford Centre for Integrative Systems Biology, University of Oxford, Oxford, United Kingdom Abstract We have conducted a protein interaction study of components within a specific sub-compartment of a eukaryotic flagellum. The trypanosome flagellum contains a para-crystalline extra-axonemal structure termed the paraflagellar rod (PFR) with around forty identified components. We have used a Gateway cloning approach coupled with yeast two-hybrid, RNAi and 2D DiGE to define a protein-protein interaction network taking place in this structure. We define two clusters of interactions; the first being characterised by two proteins with a shared domain which is not sufficient for maintaining the interaction. The other cohort is populated by eight proteins, a number of which possess a PFR domain and sub-populations of this network exhibit dependency relationships. Finally, we provide clues as to the structural organisation of the PFR at the molecular level. This multi-strand approach shows that protein interactome data can be generated for insoluble protein complexes. Citation: Lacomble S, Portman N, Gull K (2009) A Protein-Protein Interaction Map of the Trypanosoma brucei Paraflagellar Rod. PLoS ONE 4(11): e7685. doi:10.1371/journal.pone.0007685 Editor: Adam Yuan, Temasek Life Sciences Laboratory, Singapore Received September 2, 2009; Accepted October 12, 2009; Published November 3, 2009 Copyright: 2009 Lacomble et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.