a portrait of the transcriptome of the neglected trematode, fasciola gigantica—biological and biotechnological implications的画像被忽视的吸虫的转录组,小束gigantica-biological和生物技术的影响.pdfVIP

A Portrait of the Transcriptome of the Neglected Trematode, Fasciola gigantica—Biological and Biotechnological Implications 1 1 1 1 1 Neil D. Young *, Aaron R. Jex , Cinzia Cantacessi , Ross S. Hall , Bronwyn E. Campbell , Terence W. 2 3 4 5 1 Spithill , Sirikachorn Tangkawattana , Prasarn Tangkawattana , Thewarach Laha , Robin B. Gasser * 1 Department of Veterinary Science, The University of Melbourne, Werribee, Australia, 2 School of Animal and Veterinary Sciences, Charles Sturt University, Wagga Wagga, Australia, 3 Department of Pathobiology, Faculty of Veterinary, Medicine, Khon Kaen University, Khon Kaen, Thailand, 4 Department of Anatomy, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen, Thailand, 5 Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand Abstract Fasciola gigantica (Digenea) is an important foodborne trematode that causes liver fluke disease (fascioliasis) in mammals, including ungulates and humans, mainly in tropical climatic zones of the world. Despite its socioeconomic impact, almost nothing is known about the molecular biology of this parasite, its interplay with its hosts, and the pathogenesis of fascioliasis. Modern genomic technologies now provide unique opportunities to rapidly tackle these exciting areas. The present study reports the first transcriptome representing the adult stage of F. gigantica (of bovid origin), defined using a massively parallel sequencing-coupled bioinformatic approach. From .20 million raw sequence reads, .30,000 contiguous sequences were assembled, of which most were novel. Rel