有哪些信誉好的足球投注网站a novel serum metabolomics-based diagnostic approach for colorectal cancer一种新型血清metabolomics-based为结直肠癌的诊断方法.pdfVIP
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a novel serum metabolomics-based diagnostic approach for colorectal cancer一种新型血清metabolomics-based为结直肠癌的诊断方法
A Novel Serum Metabolomics-Based Diagnostic Approach for Colorectal Cancer 1 1 1 1 1 1 Shin Nishiumi , Takashi Kobayashi , Atsuki Ikeda , Tomoo Yoshie , Megumi Kibi , Yoshihiro Izumi , 1 2 2 3 1 Tatsuya Okuno , Nobuhide Hayashi , Seiji Kawano , Tadaomi Takenawa , Takeshi Azuma , Masaru Yoshida1,3,4* 1 Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Kobe, Japan, 2 Department of Clinical Laboratory, Kobe University Hospital, Kobe, Japan, 3 The Integrated Center for Mass Spectrometry, Kobe University Graduate School of Medicine, Kobe, Japan, 4 Division of Metabolomics Research, Department of Internal Medicine, Kobe University Graduate School of Medicine, Kobe, Japan Abstract Background: To improve the quality of life of colorectal cancer patients, it is important to establish new screening methods for early diagnosis of colorectal cancer. Methodology/Principal Findings: We performed serum metabolome analysis using gas-chromatography/mass-spectrom- etry (GC/MS). First, the accuracy of our GC/MS-based serum metabolomic analytical method was evaluated by calculating the RSD% values of serum levels of various metabolites. Second, the intra-day (morning, daytime, and night) and inter-day (among 3 days) variances of serum metabolite levels were examined. Then, serum metabolite levels were compared between colorectal cancer patients (N = 60; N = 12 for each stage from 0 to 4) and age- and sex-matched healthy volunteers (N = 60) as a training set. The metabolites whose levels displayed significant changes were subjected to multiple logistic regres
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