有哪些信誉好的足球投注网站a comparative proteomic analysis reveals a new bi-lobe protein required for bi-lobe duplication and cell division in trypanosoma brucei比较蛋白质组学分析揭示了一个新的bi-lobe bi-lobe复制所需的蛋白质和细胞分裂锥虫属brucei.pdfVIP
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a comparative proteomic analysis reveals a new bi-lobe protein required for bi-lobe duplication and cell division in trypanosoma brucei比较蛋白质组学分析揭示了一个新的bi-lobe bi-lobe复制所需的蛋白质和细胞分裂锥虫属brucei
A Comparative Proteomic Analysis Reveals a New Bi-Lobe Protein Required for Bi-Lobe Duplication and Cell Division in Trypanosoma brucei 1. 1. 2 1 2 2 Qing Zhou , Ladan Gheiratmand , Yixin Chen , Teck Kwang Lim , Jun Zhang , Shaowei Li , Ningshao 2 1 1 1 Xia , Binghai Liu , Qingsong Lin , Cynthia Y. He * 1 Department of Biological Sciences, National University of Singapore, Singapore, Singapore, 2 School of Life Sciences, Xiamen University, Xiamen, China Abstract A Golgi-associated bi-lobed structure was previously found to be important for Golgi duplication and cell division in Trypanosoma brucei. To further understand its functions, comparative proteomics was performed on extracted flagellar complexes (including the flagellum and flagellum-associated structures such as the basal bodies and the bi-lobe) and purified flagella to identify new bi-lobe proteins. A leucine-rich repeats containing protein, TbLRRP1, was characterized as a new bi-lobe component. The anterior part of the TbLRRP1-labeled bi-lobe is adjacent to the single Golgi apparatus, and the posterior side is tightly associated with the flagellar pocket collar marked by TbBILBO1. Inducible depletion of TbLRRP1 by RNA interference inhibited duplication of the bi-lobe as well as the adjacent Golgi apparatus and flagellar pocket collar. Formation of a new flagellum attachment zone and subsequent cell division were also inhibited, suggesting a central role of bi-lobe in Golgi, flagellar pocket collar and flagellum attachment zone biogenesis. Citation: Zhou Q, Gheiratmand L, Chen Y, Lim TK, Zhang J, et al. (2010) A Comparative Proteomic Analysis Reveals a New Bi-Lobe P
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