PCR法检测香蕉束顶病毒.PDF

福建农业学报 16( 4) :45 ～48,2001 : 1008- 0384 (2001) 04- 0045- 04 F uj ian J our nal of A g r icultur al S ciences PCR 周莉娟, 郑伟文 (, 350003) : 3 , DNA, , 4 PCR , 2 3 PCR , CTAB DNA , PCR , , 5 g BBT V : ; PCR : Q 7; S 436.67 : A Detection of banana bunchy top virus (BBTV) by PCR - , - ZH U Li juan ZHENG Wei wen (B iotech nology Center , F uj ian A cademy of A g ric ult ur al Sc iences , F uz hou , F uj ian 350003, Ch ina) Abstract: Total DNA was extracted from BBT V-infected banana leaves using three different methods, CT AB method was shown to be better than others, and the DNA templates with high quality and quantity could be obtained A protocol suitable for identifying BBTV strains isolated from Fujian province was established through. optimizing the reaction conditions, including different primers, reaction systems, PCR cycles and their combinations. Using the modified procedure, BBTV within 5g infected tissue could be detected successfully. Key words: Banana bunchy top virus PCR; , , DNA [ 1] , 20%30%, , BBTV [2] 70%80% , PCR , 1　材料与方法 (BBT V) , (P ent alonia nig roner v osa) 1. 1 ELISA , ( 12 ) 1990 BBTV , [3,4] [5] , , [ 6,7] (3 ) BBT V ELISA ELISA ,