旱生和湿生生境对蒲公英体内抗氧化物质的影响-植物研究.PDF

22 　2 　　　　　　　　　　　　　　　　　　　　　　 2002 　4 Vol.22　No.2　　　　　　　　　　　BULLETIN OF BOTANICAL RESEARCH Apr., 　2002 ＊ 宰学明　吴国荣 　龚祝南　陆长梅　刘　华　陈景耀 (, 　210097) 　　选择旱生和湿生生境中生长的蒲公英 (Tarax acum mongolicum Hand.-Mazz)分根、 叶、 花序分别测定抗氧化成分及总抗氧化能力, 并比 了SOD 同工酶谱的变化情况。结果表明 不同生境选取的蒲公英叶片中抗氧化物质以SOD、 OD、 CAT 等抗氧化酶和Vc 等小分子为主, 花序中可溶性糖、 类黄酮和绿原酸含量最高;湿生蒲公英各器官尤其是根中的SOD、 CAT 、 OD 活性高于旱生蒲公英对应器官中的酶活性, 旱生蒲公英各器官尤其是花序中的可溶性糖、 类黄酮和绿原酸含量高于湿生蒲公英对应器官中的含量;旱生和湿生生境未诱导出新的SOD 同 工酶带;旱生和湿生蒲公英对应器官的总抗氧化能力接近。 　旱生生境;湿生生境;蒲公英;总抗氧化物质;总抗氧化能力 EFFECTS ON ANTI -OXIDANT INGREDIENTS OF TARAXA CUM M ON GOLI CUM HARD.-MAZZ IN DROUGHT AND WET ENVIRONMENTS ZAI Xue-ming 　WU Guo-rong 　GONG Zhu-nan　LU Chang-mei　LIU Hua 　CHENG Jing-yao (The College of Life Science, Nanjing Normal University , Nan jing 210097) Abstract 　Anti-oxidant ingredients and total anti-oxidant capacity from Tarax acum mongolicum Hand.-Mazz collected in drought and wet environments were studied and their isozymogram from AGE were compared.Results showed that activities of anti -oxidant enzymes (eg SOD, OD, CAT etc)and content of Vc from leaves were the highest, with content of soluble sugar, flavonoid and chlorogenic acid from anthotaxies were the highes;activities of SOD, OD and CAT from or- gans of Tar ax acum mongo licum Hand.-Mazz in wet environment were higher than from corre- sponding organs in drought environment, especially from root, the contents of soluble sugar, flavonoid and chlorogenic acid in drought environment were higher than those in wet environment, especially from anthotaxies;No new SOD isozyme band could be induced by drought and wet inviron- ment;total anti-oxidant capacity