结缔组织生长因子单链抗体对博莱霉素诱导的小鼠肺纤维化的影响.docVIP

　　结缔组织生长因子单链抗体对博莱霉素诱导的小鼠肺纤维化的影响 ：苟丽霞，吴国球，范晓波，刘朝朝，吴宏斌 【摘要】 目的：研究结缔组织生长因子(CTGF)单链抗体(ScFv)对博莱霉素诱导的小鼠肺纤维化模型各阶段的支气管肺泡灌洗液(BALF)中炎症细胞数及纤维化程度的影响。方法：昆明小鼠36只，随机分为对照组、模型组和ScFv组，每组12只;后2组麻醉后气管内滴入博莱霉素制备模型，滴药后第2天开始尾静脉分别注入生理盐水或ScFv(4 mg·kg-1)，3次·周-1，对照组给予等量的生理盐水;气管内滴药后第7、14、28天分3批处死小鼠，收集左肺BALF，进行炎症细胞计数和分类;肺组织HE染色后观察肺纤维化程度。结果：(1) 第7天模型组有明显的肺泡炎，ScFv组小鼠肺泡炎症病变轻微;第28天模型组出现肺纤维化，ScFv组纤维化程度明显低于模型组(Plt;0.05);(2) 第7、14天模型组小鼠BALF中细胞总数及巨噬细胞、淋巴细胞、中性粒细胞均明显高于对照组(Plt;0.05)，ScFv组明显低于模型组(Plt;0.05)。结论：CTGF ScFv可减少肺纤维化模型BALF中的炎症细胞，减缓肺纤维化的发生 发展 。 【关键词】 结缔组织生长因子单链抗体; 肺纤维化; 支气管肺泡灌洗液; 炎症细胞; 小鼠 　　[Abstract] Objective: To investigate the effects of the singlechain variable fragment of antibody(ScFv) against connective tissue gromatory cells in bronchoalreolar lavage fluid(BALF) and fibrosis dgree of pulmonary fibrosis model induced by bleomycin. Methods: Thirty six male Kunming mice ly divided into three groups: the control group, the model group and the ScFv group. The latter tycin via trachea(5 mg·kg-1) and the control injected g·kg-1), and the others onary tissue ice in each group inistration to collect samples，onary lobe icroscope. Results: (1) Apparent alveolitis appeared in the model group, uch slight in the ScFv group at the 7th day. At the 28th day, both of the groups appeared pulmonary fibrosis, but the fibrosis of ScFv group seemed in a earlier stage(Plt;0.05). (2) At the 7th and 14th day, the number of cell and the propotion of neutrophils in BALF in the model group ber of cells and the propotion of neutrophils in BALF in the ScFv group odel group(Plt;0.05). Conclusion: ScFv can decrease the number of inflammatory cells in BALF of the pulmonary fibrosis model induced by bleomycin, ent of antibody against connective tissue groonary fibrosis; bronchoalveolar lavage fluid; inflammatory cells; mice 　　肺纤维化是肺损伤后常见的反应和并发症，尽管致病因素不同，但其主要的病理改变为弥漫性肺泡炎症、肺成纤维细胞灶的形成以及细胞外基质的反复修复和过度沉积[1]。肺纤维化常以呼吸功能永久丧失为最终结局，目前尚缺乏有效防治手段[2]。肺纤维化的早期为肺泡炎阶段，大量的炎症细胞和肺组织细胞分泌多种细胞因子和酶，构成一个相互交错的 网络 ，参与肺纤维化的形成，逐渐发展为弥漫性的肺纤维化，转化生长因子(