RP-HPLC法测定黄芩苷及黄芩素含量.docVIP

RP-HPLC法测定黄芩苷及黄芩素含量摘要：目的用反向高效液相（RP-HPLC）法测定滇黄芩和黄芩中黄芩苷和黄芩素的含量。方法乙腈（含1.0 %甲酸）-1.0 %甲酸溶液为流动相，梯度洗脱，流速为1.0 mL/min，检测波长274 nm，柱温30 ℃。结果在精密度，重现性，稳定性实验中，保留时间和峰面积的RSD均小于2%；加标回收率分别为100.7%和100.5%，RSD为1.78% 和1.85 %；黄芩苷在0.102~2.04μg之间，黄芩素在0.72~14.40 μg之间呈良好的线性关系，r=0.999 9。结论此实验方法简便、准确、重复性好。 关键词：黄芩苷；黄芩素；反相高效液相；含量测定 中图分类号：O657.7+2文献标识码：A文章编号：1672-979X(2007)03-0015-03 Determination of Baicalin and Baicalein by RP- HPLC XIONG Chun-mei1, GUO Ya-dong1*, MA Yin-hai2, LIAO Yu1, ZHANG Ping1 (1.School of Pharmacy, Kunming Medical College, Kunming 650031,China；2. Department of Chemistry, Kunming Normal College, Kunming 650031, China) Abstract：Objective To establish a RP-HPLC method for the determination of the contents of baicalin and baicalein in Scutellariae. Methods Zorbax 250 mm×4.6 mm i.d.5.0 μm column was used with a mixture liquid of acetonitrile (containing 1.0 % formic acid)- 1.0 % formic acid as mobile phase in gradient elution. The column temperature was controlled at 30℃, the detection wavelength was 274 nm. Results In the experiments of precision, reproducibility and stability, the samples could be separated entirely. The average recoveries of the baicalin and baicalein were respectively 100.7% and 100.5% with RSD of 1.78% and 1.85%. The linear ranges were 0.102~ 2.04 μg (r=0.999 9) for baicalin and 0.72~14.40 μg (r=0.999 9) for baicalein. Conclusion This method is simple, accurate and has a good reproducibility for the determination of the contents of baicalin and baicalein in Scutellariae. Key words：baicalin; baicalein; RP-HPLC; assay 黄芩是常用中药，《中华人民共和国药典》（一部）收录的黄芩为唇形科植物黄芩（Scutellaria baicalensis Georgi ）的干燥根[1]，市场流通的商品黄芩除来自野生的和栽培的黄芩之外，有些地方还将根断面为黄色的黄芩属（Scutellaria）的多种植物作黄芩药用，主要有黏毛黄芩（S.viscidula Bunge）、和滇黄芩（S.amoena C.H.Wright）等。滇黄芩是西南地区药用黄芩的主流品种，分布于云南、贵州等省，资源丰富，最早记载于《滇南本草》。黄芩苷和黄芩素具有多种药理活性，在药物中的应用广泛，用量大[2]。用HPLC法测定黄芩中黄芩苷的含量多有报道[3-6]，本实验用RP-HPLC法对云南8处产的滇黄芩与《中华人民共和国药典》收录的黄芩中的黄芩苷和黄芩素含量进行测定比较，结果表明，滇黄芩饮片与其他黄芩饮片中有效成分的含量差别不明显。 1 仪器与试药 Agilent 1100高效液相色谱仪（