GC法测定麝香祛风湿膏中薄荷脑、丁香酚等组分含量.docVIP

GC法测定麝香祛风湿膏中薄荷脑、丁香酚等组分含量摘要：目的建立气相色谱法同时测定麝香祛风湿膏中桉油精、樟脑、冰片、薄荷脑、水杨酸甲酯、桂皮醛、丁香酚等7组分的含量。方法用挥发油提取法提取样品中的挥发性成分制备供试品溶液，以石英毛细管柱（安捷伦 DB-WAX，长15 m，内径0.53 mm，涂层0.5μm）为色谱柱，氮气为载气，FID检测器的温度为250 ℃，柱温为程序升温。结果在该色谱条件下桉油精、樟脑、冰片、薄荷脑、水杨酸甲酯、桂皮醛、丁香酚的分离度和线性关系良好，平均回收率（n＝6）分别为98.7 %（RSD＝1.1 ％）、99.0 %（RSD＝1.0 ％）、99.4 %（RSD＝0.8 ％）、99.7 %（RSD＝0.4 ％）、99.6 %（RSD＝0.5 ％）、99.5 %（RSD＝0.8 ％）、99.5 %（RSD＝0.9 ％）。结论本法简便快捷，准确性和重复性均好，可用于麝香祛风湿膏的质量控制。 关键词：薄荷脑；水杨酸甲酯；丁香酚；麝香祛风湿膏；气相色谱法；含量测定 中图分类号：R914.1文献标识码：A文章编号：1672-979X(2008)09-0051-03 Determination of Menthol, Eugenol and Other Constituents in Shexiang Qufengshi Adhesive Plasters by GC Method YANG Jing-jing1, GUO Jian-gu 1, LIU Xian-yang 1, ZHANG Xiao-shan 2 (1. Shantou Wuhuan Pharmacy Factory Co., Ltd, Shantou 515041, China; 2. The Advanced Instrument Analysis Testing Center, Shantou University, Shantou 515041, China) Abstract：Objective To determine the contents of eucalyptol, camphor, synthetic borneol, menthol, methyl salicylate, cinnamaldehyde and eugenol in Shexiang Qufengshi Adhesive Plasters by GC method. Methods The sample solution was prepared by distillation with an essential oil collector. The GC system consisted of a quartz capillary column（Agilent DB-WAX, 15 m, 0.53 mm, 0.5 μm） as stationary phase, nitrogen as carrier gas, FID detector at 250 ℃ and programmed heating method. Results The resolution and linearity of eucalyptol, camphor, synthetic borneol, menthol, methyl salicylate, cinnamaldehyde and eugenol were good with the recovery rate of eucalyptol at 98.7 % (RSD=1.1 %) camphor at 99.0 % (RSD=1.0 %), synthetic borneol at 99.4 % (RSD=0.8 %), menthol at 99.7 % (RSD=0.4 %), methyl salicylate at 99.6 % (RSD=0.5 %), cinnamaldehyde at 99.5 % (RSD=0.8 %), eugenol at 99.5 % (RSD=0.9 %), respectively. Conclusion This method is convenient, rapid, accurate and reproducible, and can be used for the quality control of Shexiang Qufengshi Adhesive Plasters. Key words：menthol; methyl salicylate; eugenol; Shexiang Qufengshi Adhesive Plas