实验环境和人体表面微生物（Laboratory environment and human surface microorganisms）.doc

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实验环境和人体表面微生物（Laboratory environment and human surface microorganisms）.doc

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实验环境和人体表面微生物（Laboratory environment and human surface microorganisms）

实验环境和人体表面微生物（Laboratory environment and human surface microorganisms） Detection of laboratory environment and human body surface microorganisms Abstract: universities pay more and more attention to the training of students practical ability of the students in the laboratory More and more room and opportunities. However, different from the laboratory microbiology laboratory in other disciplines, the Right is a living creature, but also have different degrees of infection. This experiment project The significance is to make students recognize themselves around the existence of microorganisms from the senses, master of microorganisms Isolation, inoculation technology. On the enrichment of microorganisms to do sterilization, microbial The laboratory safety management standardization. Keywords: Laboratory; microbial count; Materials and methods: 1 1.1 experimental materials 1.1.1 material Ingredients: potato agar (bought); NaOH (pure Tianjin Kaitong chemical analysis) Reagent Co. Ltd.; Tianjin inbor beef extract peptone and biochemical reagent Co. Ltd.; Tianjin Kaitong Chemical Reagent Co., Ltd.; sucrose (AR) Shanghai Sinopharm chemical test Agent Co. Ltd. 1.1.2 main equipment and reagents 1.1.2.1 main equipment High pressure steam sterilizer, clean bench, electronic balance, incubator, electric thermostatic drying box, mold incubator, ultrasonic cleaner, pipette, shake culture box, a stereomicroscope. Medium plate, sterile water, sterilized cotton swab (in vitro), inoculating loop, test tube rack, alcohol lamp, alcohol bottle, mark pen, waste cylinder, toothpick. 1.1.2.2 Kit Iodine solution, xylene, 95% ethanol, cedar oil, methylene blue, crystal violet, carbolfuchsin, saffron, malachite green, blue cotton etc. 1.1.3 separation medium count Potato medium (PDA) ratio is: 200 g, 20 g of potato sucrose agar, 20 g, 1000 ml water. Beef extract peptone medium ratio: beef extract 3 G, protein 10g NaCl 5g, 20 g, 1000 ml water agar. The 1.1.4 experiments were conducted on the fo