3种ELISA试剂盒检测不同亚型外源性鸡白血病病毒的比较_郭慧君.pdfVIP

　2010, 41(3):310-314 A cta Veterinari a et Zoo techn ica S in ica 3 ELISA 郭慧君,李中明,李宏梅, 柴家前,马诚泰, 王洪进, 崔治中＊ (, 271018) 　:、(A LV), A (A LV-A)、C (AL V-C)2 J (AL V-J-P Y A LV-J-WS)(AL V)、、(100 、10、1 μL)3 DF 1 , A、B、C 3 ELISA A LV (P27 )。 , A LV-A A LV-J-PY, , A 3 ;, 7 。 B , 2 (7 5 ; 15 13 ); C , ( 13 )。A LV-C A LV-J-WS , A , 5 9 , 、 15 d ;B、C 2 3 。, 3 ELISA 3 A LV , A , ;B 。, A LVs 。AL V 。 :(A LV );(ELISA );;DF 1 :S852.659.3;S858.3 15.3 　　　　:A 　　　　:0366-6964(20 10 )0 3-0 310-05 Comparision of Dynamic Detection with Three ELISA Kits to Different Sub roups of Exosomic ALVs in DF1 Cells GUO H ui-un, LI Zhong-ming, LI Hong-mei, CH AI Jia-qian, MA Cheng-tai, ＊ WAN G Hong-in, CU I Zhi-zhong (College of Veterinary Med icine,S handong Agr icultura l University , Tai ′an 271018, Ch ina) Abstract:DF 1 cel ls w ere inoculated w ith three dif ferent doses (100, 10, 1 μL )o f A LV -A, A LV - C, A LV -J-PY and A LV -J-WS separated f rom diff erent chickens to ev aluat e three ki nds o f A, B, C ELISA kit s.T he sam ples o f DF 1 cel ls supernat ant w ere obt ai ned at dif ferent day s post inocula- tion (dpi)and detected w it h three kinds of kits aft er f reezing and t haw ing o nce .T he result s indi- cated t hat t he samples inoculated w ith A LV -A o r A L V-J-P Y we re bo th det ect ed positively firstly at t he 3rd dpi w it h A ELISA kit fo r 100 μL inoculation doses and at the 7t h dpi fo r 1 μL dose;If with B E LISA kit, tw o A LV s of subg roup w ere det ected posit ively w it hin some day s po st ino cu - la tion, but t he detectable t ime w ere longer than t hat of A EL ISA kit;If with C EL ISA kit