液体菌种培训教材(三)液体菌种的制作方法（Liquid spawn training material (three) method for making liquid spawn）.doc

液体菌种培训教材(三)液体菌种的制作方法（Liquid spawn training material (three) method for making liquid spawn） Liquid spawn training materials (excerpt three) April 10, 2010 (three) the method of making liquid strains The liquid spawn production methods can be divided into electromagnetic stirring method, shake flask method, simple fermentation machine (oxygen) method and fermentation method. 1., electromagnetic mixing method is relatively simple, investment is relatively small, 200 yuan of investment can be produced. It takes 7 days to grow from the inoculum to the mycelium. But there are also shortcomings in the production of small, so the production of several agitators are often produced simultaneously to solve. It can also be solved by dilation. The main production process: in sterile conditions, test tube into the triangle bottle, stirring for 4-7 days to get a large number of bacteria. 2., shake bottle machine is also a relatively simple method, vaccination simple, convenient management, production according to the machine settings and different. The investment will be 1000-3000 yuan. But there are also many disadvantages such as noise. Production process: in sterile conditions, test tube into a bottle, shake. Generally, 7 days or so, there will be a large number of balls. 3. simple submerged fermentation (oxygen) method is relatively cumbersome, is mainly connected to the equipment carried out under sterile conditions, if part of the problem which can not guarantee the success rate. Investment should also be more than 3000 yuan. Production process: in sterile conditions, the tube was received into the container, ventilated for 7 days, get a lot of balls. 4. fermentation tank method is the most demanding one, involving many aspects of knowledge, it is difficult to self-study, and investment is also high, generally more than 10000 yuan. If you have a large amount of production or a culture field, it is better to use a fermenter. Because of the large amount of production, you wil