烟草青枯病苗期抗性鉴定的漂浮池恒温水培接种法研究-中国烟草科学.PDF

2013-0434 2 Chinese Tobacco Science 45 1,2 1* 2 1 1 [1. 653100 2. 650201] 7 S572.08 1007-5119201302-0045-04 DOI 10.3969/j.issn.1007-5119.2013.02.010 Floating Pool Water Inoculation Method for Tobacco Resistance Identification to Bacterial Wilt 1,2 1* 2 1 1 XU Wen , LIU Yong , JI Guanghai , LU Xiuping , LI Yongping (1. Yunnan Academy of Tobacco Agricultural Sciences, China Tobacco Breeding Research Southern Center, Yuxi, Yunnan 653100, China; 2. College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China) Abstract: Floating pool system for screening bacterial wilt resistance of tobacco seedlings was developed by building temperature controlled water heating float pool and inoculating with pool water in greenhouse. The resistance result of seven known tobacco varieties evaluated by floating pool water inoculation method was consistent with the varieties inherent resistance levels. The advantages of floating pool temperature controlled water inoculation method are rapid disease development, small space needed, less soil-borne disease disturbance, and high result repetition. Keywords: tobacco bacterial wilt; floating pool; water inoculation; resistance identification Ralstonia [4-7] solanacearum [1-2]