牛磺酸在重型颅脑创伤裸鼠体内分布的初步研究.docVIP

牛磺酸在重型颅脑创伤裸鼠体内分布的初步研究 王 伟1，黄慧玲2，杨 铭3，武俏丽2，温晓昶1，宋 婷1，姚 鑫2 （300070天津，天津医科大学研究生院1；300060天津，天津市环湖医院研究所2；300020天津，中国医学科学院血液病3） [摘要] 目的 应用小动物活体成像技术观察牛磺酸在重型颅脑创伤裸鼠体内的代谢及分布变化。 方法 采用Cy7荧光染料标记牛磺酸（Cy7-Tau），选择雄性SFP级BALB/C裸鼠32只，随机分为正常（normal）组、假手术（sham）组、脑创伤染料（TBI）组、牛磺酸治疗（Tau）组，每组8只。采用液压打击法在裸鼠左大脑半球制作颅脑创伤（TBI）模型，normal组及sham组、Tau组伤后立即尾静脉注射Cy7-Tau 5 mg/kg，TBI组伤后尾静脉注射游离Cy7：5 mg/kg。分别于给药后1、2、4、8、12、24 h行活体成像动态观察裸鼠体内Cy7-Tau、Cy7代谢及分布变化，每个时间点每组处死1只裸鼠，离体脑、心脏、肝脏行荧光成像。 结果 ①正常状态下，经静脉注射后，Cy7-Tau立即全身分布，一过性地在脑、心脏、肝脏富集，大部分24 h内即可通过肾脏经膀胱排泄；给药后4 h，Cy7-Tau与胆汁酸结合生成胆盐，并长时间存在于胆囊；②Cy7-Tau在脑创伤裸鼠体内特异性地向脑创伤区域富集，4 h达高峰，至伤后24 h仍大量存在；裸鼠头部切口皮缘有Cy7-Tau富集，并可持续至伤后24 h；③静注Cy7后弥漫分布于脑创伤裸鼠皮下，组织器官内无特异性富集。 结论 活体成像技术可以实时监测Cy7-Tau在颅脑创伤裸鼠体内的动态分布，Tau通过肾脏排泄，在脑创伤、炎症部位特异性富集并长时间存在。 [关键词] 活体成像；牛磺酸；颅脑创伤；组织分布；代谢 [中图分类号reliminary study of taurine biodistribution in nude mouse with severe traumatic brain injury Wang Wei1, Huang Huiling2, Yang Ming3, Wu Qiaoli2, Wen Xiaochang1 , Song Ting1 , Yao Xin2 (1Graduate School of Tianjin Medical University, Tianjin,300070; 2Research Institution of Tianjin Huanhu Hospital, Tianjin,300060; 3Chinese Academy of Medical Sciences Institute of Hematology , Tianjin,300020, China) [Abstract] Objective To conduct an in vivo optical imaging analysis of the metabolism and change of biodistribution of taurine in nude mouse with severe traumatic brain injury. Methods Cy7 fluorescent dye was applied to label taurine，eighteen BALB/C nude mice were randomly divided into normal group(group normal), sham operation group (group sham)，brain trauma group (group TBI) and taurine treatment group(group Tau) equally．TBI models were made in left brain of nude mouse by hydraulic shock，while sham group only opened bone window．Mice in group sham,Tau were given Cy7-Tau 5 mg/kg immediately after injury injected via tail vein; Group normal was given Cy7-Tau 5 mg/kg directly and group TBI was given the same amount of Cy7.In vivo optical imaging system was used to observe the metabolism and change of biodistribu