三种药物对饲服TCDD的胎鼠腭突超微结构的影响.docVIP

三种药物对饲服TCDD的胎鼠腭突超微结构的影响 何晓梦 刘翠苹 袁心刚 魏光辉 傅跃先( 400014 重庆,重庆医科大学附属儿童医院整形外科，儿童发育疾病教育部重点实验室，重庆市儿科学重点实验室，重庆市（儿童发育重大疾病诊治与预防）国际科技合作基地【摘要】目的：观察叶酸、白藜芦醇和α-萘黄酮拮抗四氯二苯二噁英(23,7,8- tetrachlorodibenzo-p-dioxin,TCDD)诱导胎鼠腭裂发生的效应，及对饲服TCDD的胎鼠腭突表面超微结构的影响。方法：妊娠第10天孕鼠随机分组，每组8只。TCDD组以TCDD 28μg/kg灌胃，对照组以玉米油0.1ml灌胃，叶酸组以叶酸10 mg/kg + TCDD28μg/kg灌胃、白藜芦醇组以白藜芦醇50mg/kg + TCDD28μg/kg 灌胃、α-萘黄酮组以α-萘黄酮5mg/kg+TCDD28μg/kg灌胃，于妊娠第17.5天在解剖显微镜下观察胎鼠腭裂的发生率。另取15只孕鼠随机分组，每组3只，处理方法同前，分别于妊娠第13.5、14.5和15.5天剪取胎鼠头部，扫描电镜观察腭突表面的超微结构。结果：TCDD组胎鼠腭裂发生率为92.86%，对照组未见胎鼠腭裂形成。叶酸组、白藜芦醇组和α-萘黄酮组胎鼠腭裂发生率分别为73.08%74.51% 、65.52%，与TCDD组相比，差异有统计学意义（P0.05）。TCDD组腭中嵴上皮细胞表面逐渐破裂、皱缩，伪足结构逐渐减少、消失，最终形成腭裂；对照组、叶酸组、白藜芦醇组和α-萘黄酮组腭中嵴上皮细胞表面光滑整齐、饱满膨胀，表面丝状伪足逐渐增多、延长，一直持续到腭突融合结束。结论：叶酸、白藜芦醇和α-萘黄酮均能恢复小鼠腭中嵴上皮细胞表面的超微结构，从而拮抗TCDD的致腭裂效应。 【关键词】叶酸；白藜芦醇；α-萘黄酮；腭裂；TCDD；扫描电镜 【中图分类号】R114 【文献标志码】A Effects of three drugs on mouse embryonic palate ultrastructure after feeding TCDD HE Xiaomeng, LIU Cuiping , YUAN Xingang, WEI Guanghui, FU Yuexian.（Ministry of Education Key Laboratory of Child Development and Disorders ;Key Laboratory of Pediatrics in Chongqing;Chongqing International Science and Technology Cooperation Center for Child Development and Disorders,Chongqing 400014, China） 【Abstract】Objective To observe the incidence and ultrastructure of folic acid, resveratrol and α-naphthoflavone antagonize TCDD- induced fetal mice cleft palate. Methods On gestation day 10(GD 10), 40 pregnant mice were randomly assigned to 5 groups. TCDD group(TCDD 28μg/kg), control group(corn oil 0.1ml/kg), folic acid group (folic acid 10mg/kg + TCDD 28μg/kg), resveratrol group (resveratrol 50mg/kg + TCDD 28μg/kg), α-naphthoflavone group (α-naphthoflavone 5mg/kg + TCDD 28μg/kg) were gavaged. To observe the incidence of cleft palate of each group on GD 17.5. Another 15 pregnant mice were randomly divided into five groups,same as above.The head coronal sections of the fetal mice were prepared at GD 13.5,14.5 and 15.5, observed by Scanning Electron Microscopy(SEM). Results The inc