DNA双链断裂损伤修复系统研究进展.pdfVIP

2007 38 4 295 综　　述 * DNA △ 黄　敏　缪泽鸿　 丁　健 ( , 201203) 　多种内源或外源因素都能造成细胞基因组DNA 损伤,细胞内建立了复杂的修复系统来应对 不 形式的损伤。其中DNA 双链断裂(DNA double-strand breaks, DSBs)作为最严重的损伤形式, 主要激活 源重组修复(Homologous recomb ination repair)和非 源末端连接(N on-homologous end joining)通路。这两条通路都是由多 个修复元件参与、经过多步反应的复杂过程。两者各具特点、 协 作用, 共 维护细胞基因组的稳定性。对其分子机制的阐明为肿瘤放化疗的辅助治疗提供了 潜在的作用靶点。 　DNA双链断裂;修复通路; 源重组修复;非 源末端连接 　R9 R epa ir Pathway s in R esponse to DNA Doub le-Strand B reaks　HUANG M in, M IAO Ze-Hong, DING Jian (D ivision of An ti-tum or P harm aco logy, S ta teK ey Labora tory of D rug R esearch, Shangha i Institu te of M a teria M ed ica, Ch ineseA cad emy of S ciences, Shanghai 201203, China) Ab stract　DNA double-strand breaks (DSBs)are the principal cytotoxic lesions caused bym any exoge- nous and endogenous factors. In response to DSBs, cells have evolved complex and h ighly conserved sy-s tem s, wh ich m ainly consist of homologous recombination repair (HR)and non-homologous end joining (NHEJ)pathw ays, to effectively repair the lethal lesions. The two pathw ays play crucial roles in prese-r ving the genom ic integrity. Here, w e provide an overview of detailed process, concerned molecu les and regu latory factors in these pathw ays. A ccumu lated know ledge of DSBs repair may offer opportun ities to develop more effective treatments for cancer. K ey w ords　DNA double-strand breaks;repair pathw ays;homologous recombination repair;non-homo-l ogous end joining 　　DNA , ;, 、DNA 、、 。 、