基于纳米金修饰丝网印刷电极的乙醇生物传感器传感技术学报.PDF

第 22 卷 　第 12 期 传 感 技 术 学 报 Vol . 22 　N o . 12 2009 年 12 月 CHIN ES E J OU RNAL OF S EN SORS AND AC TUA TORS Dec . 2009 Study of Alcohol Biosensor Based on ScreenPrinted Carbon Electrodes Modif ied by Gol d Nanoparticles C H E N Z h u l i , GUO X is h an , Z H U S ong m i ng Col leg e of B iosy s tems Eng i neeri ng an d Food S cience , Z hej i ang Uni vers ity , H ang z hou 3 10029 , Chi na Abstract :A dispo sable alcohol dehydrogenase bio sensor for detection of et hanol has been developed. It is p repared by immobilizing alcohol dehydrogenase on t he surface of screenp rinted carbon electrode modified by gold nanopar ticles ,while potassium ferricyanide is employed as electron transfer mediator . The comp arative experimental result s show t hat t he gold nanoparticles modification sp eeds up t he electrode reaction and imp roves t he sensitivities of the μ sensor s. The alcohol bio sensor s response time is only about 25 s ,and t he sensitivity of t he sensor s is 006 A (mmol/ L) - 1 ,while t he measurable linear concentration range is 10 mmol/ L to 10 mmol/ L . Key words :gol d nanop article s ;alcohol dehydro gena se ; screenp rint ed car bon elect ro de s ;bio sen sor EEACC :7230J 基于纳米金修饰丝网印刷电极的乙醇生物传感器 陈珠丽 ,郭希山 ,朱松明 浙江大学生物系统工程与食品科学学院 ,杭州 3 10029 摘 　要 :在丝网印刷电极上利用吸附法固定乙醇脱氢酶 ,并用纳米金进行修饰 , 以铁氰化钾为介体制作了用于酒精检测的一 次性乙醇脱氢酶电极试纸 。纳米金颗粒修饰酶电极 ,极大地改善了电极电流响应 ,提高了传感器的灵敏度 。此乙醇传感器的 μ ( ) - 1 响应时间仅为 25