可触摸低温等离子体对硅胶手的消毒灭菌实验研究.pdf

生物医学工程学杂志 2017年4月第34卷第2期 • 303 •   ·新技术与新方法· 可触摸低温等离子体对硅胶手的 消毒灭菌实验研究 1 1 2 1 2 2 郑超 ，寇艳芹，刘振，姜红强，黄逸凡，闫克平 1. 浙江省医疗器械不良事件监测和安全研究中心（杭州  310009 ） 2. 浙江大学 化学工程与生物工程学院（杭州  310027 ） 【摘要】   本文采用自主研制的可触摸式低温等离子体系统，研究了等离子体对硅胶手表面的消毒灭菌效 果，以模拟和评估对人手的处理。等离子体处理硅胶手表面的金黄色葡萄球菌、大肠杆菌、白色葡萄球菌和铜绿 假单胞菌时，并无显著差异。等离子体的消毒灭菌效率随时间的延长而提高，随细菌初始密度的增高而降低。细 6 7 菌初始数量为 1.0×10 ～1.0×10 CFU 时，灭菌过程主要发生在前 5 s ，灭菌率在 99% 以上。本研究为研制可用于人 手的新型等离子体设备奠定了基础。 【关键词】  低温等离子体；等离子体医学；消毒灭菌；手消毒 Experimental study on silicone hand disinfection by a touchable non-thermal plasma 1 1 2 1 2 2 ZHENG Chao , KOU Yanqin , LIU Zhen , JIANG Hongqiang , HUANG Yifan , YAN Keping 1. Zhejiang Center for Medical Device Adverse Event Monitoring and Safety Research, Hangzhou 310009, P.R.China 2. College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, P.R.China Corresponding author: LIU Zhen, Email: zliu@zju.edu.cn 【Abstract 】 In this study, silicone hand was disinfected by homemade touchable non-thermal plasma to simulate and evaluate its feasibility of application on human hand. Experimental results showed that there was no significant difference when Escherichia coli (E. coli), Staphylococcus aureus (S. au), Staphylococcus albus (S. al), and Pseudomonas aeruginosa (P. ae) were loaded on the silicone hand surface and treated with plasma. The efficiency of plasma disinfection was higher when the treatment time was prolonged or initial bacterial density was lower. When initial bacterial number 6 7 was 1.0×10 –1.0×10 CFU, the plasma disinfection process mainly occured in